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[猪增生性肠炎诊断的文献综述]

[Literature review of the diagnosis of porcine proliferative enteropathy].

作者信息

Nathues Heiko

机构信息

Aussenstelle für Epidemiologie, Stiftung Tierärztliche Hochschule Hannover.

出版信息

Berl Munch Tierarztl Wochenschr. 2007 Nov-Dec;120(11-12):499-507.

Abstract

Lawsonia (L.) intracellularis is the etiologic agent of ileitis, which occurs in different forms in pigs. The infection can be demonstrated by several methods. In routine diagnosis the indirect detection of L. intracellularis is conducted by measuring antibodies performing an indirect IFT or ELISA. Furthermore, an IFT and PCR on faecal and/or tissue samples are well established for the direct detection. The suitability of PCR on faecal samples is frequently discussed. Problems are related to various inhibitors that are component of faeces. When interpreting laboratory results, the past medical history of pigs, i.e. antimicrobial treatment, has to be considered. Since 2004 a new vaccine against ileitis is licensed. A possible influence of vaccination on diagnostic testing, especially PCR and ELISA, is hardly investigated. Therefore, the interpretation of laboratory results from vaccinated animals is, considering the current literature, restrained.

摘要

胞内劳森菌(Lawsonia, L.)是猪回肠炎的病原体,猪回肠炎有多种不同形式。感染可通过多种方法进行检测。在常规诊断中,通过检测抗体进行间接免疫荧光试验(IFT)或酶联免疫吸附测定(ELISA)来间接检测胞内劳森菌。此外,针对粪便和/或组织样本进行IFT和聚合酶链反应(PCR)以进行直接检测的方法也已成熟。粪便样本PCR检测的适用性经常被讨论。问题与粪便中的各种抑制剂有关。在解释实验室结果时,必须考虑猪的既往病史,即抗菌治疗情况。自2004年以来,一种针对回肠炎的新型疫苗已获许可。疫苗接种对诊断检测(尤其是PCR和ELISA)的可能影响几乎未得到研究。因此,根据现有文献,对接种疫苗动物的实验室结果的解释受到限制。

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