Leite Fernando L, Winfield Brittanie, Miller Elizabeth A, Weber Bonnie P, Johnson Timothy J, Sylvia Fred, Vasquez Erika, Vannucci Fabio, Beckler Dana, Isaacson Richard E
Boehringer Ingelheim Animal Health USA Inc., Duluth, GA, United States.
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.
Front Vet Sci. 2021 Jul 16;8:692521. doi: 10.3389/fvets.2021.692521. eCollection 2021.
Porcine proliferative enteropathy remains one of the most prevalent diseases in swine herds worldwide. This disease is caused by , an intracellular bacterial pathogen that primarily colonizes the ileum. In this study, we evaluated changes to the microbiome of the ileal mucosa, ileal digesta, cecal digesta, and feces subsequent to challenge with and to an oral live vaccine against . Given that gut homogenates have been used since 1931 to study this disease, we also characterized the microbial composition of a gut homogenate from swine infected with that was used as challenge material. The challenge led to a dysbiosis of the microbiome of both the small and large intestine marked by an increase of pathobionts including , and . This microbiome response could play a role in favoring colonization and disease as well as potentially predisposing to other diseases. Vaccination altered both small and large intestine microbiome community structure and led to a significant 3.03 log reduction in the amount of shed by the challenged pigs. Vaccination also led to a significant decrease in the abundance of , and among other microbial changes compared with non-vaccinated and challenged animals. These results indicate that infection is associated with broad changes to microbiome composition in both the large and small intestine, many of which can be mitigated by vaccination.
猪增生性肠炎仍然是全球猪群中最普遍的疾病之一。这种疾病由一种细胞内细菌病原体引起，该病原体主要定殖于回肠。在本研究中，我们评估了在用该病原体和针对该病原体的口服活疫苗进行攻毒后，回肠黏膜、回肠食糜、盲肠食糜和粪便微生物群的变化。鉴于自1931年以来一直使用肠道匀浆来研究这种疾病，我们还对用作攻毒材料的感染该病原体的猪的肠道匀浆的微生物组成进行了表征。该病原体攻毒导致小肠和大肠微生物群失调，其特征是包括该病原体在内的致病共生菌增加。这种微生物群反应可能有助于该病原体的定殖和疾病发生，以及可能易患其他疾病。接种疫苗改变了小肠和大肠微生物群的群落结构，并使攻毒猪排出的该病原体数量显著减少3.03个对数。与未接种疫苗和攻毒的动物相比，接种疫苗还导致该病原体以及其他微生物变化的丰度显著降低。这些结果表明，该病原体感染与小肠和大肠微生物群组成的广泛变化有关，其中许多变化可通过接种疫苗得到缓解。
