De Almeida Danielle Nogueira, Da Silva Oliveira Rodrigo, Brazil Beatriz Gomes, Soares Maurilio José
Laboratório de Biologia Celular de Microrganismos, Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Manguinhos, 21045-900 Rio de Janeiro, Brasil.
J Med Entomol. 2004 Sep;41(5):819-25. doi: 10.1603/0022-2585-41.5.819.
The patterns of exochorion ornaments on eggs of seven South American Lutzomyia sand fly species were analyzed by scanning electron microscopy (SEM): Lutzomyia (Lutzomyia) cruzi (Mangabeira 1938), Lutzomyia (Micropygomyia) evandroi (Costa Lima and Antunes 1936), L. (Nyssomyia) intermedia (Lutz and Neiva 1912), L. longipalpis (Lutz and Neiva 1912), L. migonei (Franca 1920), L. (Nyssomyia) neivai (Pinto 1926), and L. renei (Martins, Falcao, and Silva 1957). Different patterns were observed, which showed the distinction between some species. Egg ornaments in L. cruzi and L. longipalpis appear as single, parallel, unconnected ridges, whereas eggs of L. migonei appear as single, parallel, connected ridges. Eggs of L. (Nyssomyia) intermedia and L. (N.) neivai present a new variation of the single, unconnected, parallel ridges pattern: small tubercles are present, distributed between the ridges. Eggs of L. renei present an elliptical pattern, with most structures connected by straight ridges. Eggs of L. (M.) evandroi present a polygonal pattern, with alternate rows of small and large hexagons. Our data emphasize the advantages of the SEM approach in the study of the exochorion patterns of Lutzomyia eggs and in the distinction of the sand fly species.
通过扫描电子显微镜（SEM）分析了七种南美白蛉属沙蝇卵的外卵壳纹饰模式：克氏白蛉（Lutzomyia (Lutzomyia) cruzi，Mangabeira于1938年命名）、埃氏白蛉（Lutzomyia (Micropygomyia) evandroi，Costa Lima和Antunes于1936年命名）、中间白蛉（L. (Nyssomyia) intermedia，Lutz和Neiva于1912年命名）、长须白蛉（L. longipalpis，Lutz和Neiva于1912年命名）、米氏白蛉（L. migonei，Franca于1920年命名）、内氏白蛉（L. (Nyssomyia) neivai，Pinto于1926年命名）和雷氏白蛉（L. renei，Martins、Falcao和Silva于1957年命名）。观察到了不同的模式，这些模式显示了一些物种之间的区别。克氏白蛉和长须白蛉的卵纹饰表现为单一、平行、不相连的脊，而米氏白蛉的卵表现为单一、平行、相连的脊。中间白蛉和内氏白蛉的卵呈现出单一、不相连、平行脊模式的一种新变体：在脊之间分布有小瘤。雷氏白蛉的卵呈现出椭圆形模式，大多数结构由直脊相连。埃氏白蛉的卵呈现出多边形模式，有大小交替的六边形排。我们的数据强调了SEM方法在研究白蛉属卵的外卵壳模式以及区分沙蝇物种方面的优势。
