使用二维魔角旋转核磁共振技术对具有不同均匀标记的蛋白质混合物进行分子界面探测。
Probing molecular interfaces using 2D magic-angle-spinning NMR on protein mixtures with different uniform labeling.
作者信息
Etzkorn Manuel, Böckmann Anja, Lange Adam, Baldus Marc
机构信息
Max-Planck-Institute for Biophysical Chemistry, Department of NMR-based Structural Biology, Am Fassberg 11, 37077 Göttingen, Germany.
出版信息
J Am Chem Soc. 2004 Nov 17;126(45):14746-51. doi: 10.1021/ja0479181.
A general NMR strategy to directly study molecular interfaces under magic angle spinning is introduced. The approach is based on the spectroscopic analysis of uniformly, but heterogeneously, labeled molecular mixtures containing the spin species X and Y (X:Y). For the case of an ((15)N:(13)C) labeled sample, the use of NC, NHC, and NHHC transfers is demonstrated. Applied to the ((13)C:(15)N) labeled dimeric form of the 85 amino acid protein Crh, the NHHC approach reveals a variety of monomer-monomer interactions in the microcrystalline state.
介绍了一种在魔角旋转下直接研究分子界面的通用核磁共振策略。该方法基于对含有自旋物种X和Y（X:Y）的均匀但非均匀标记的分子混合物进行光谱分析。对于（（15）N:（13）C）标记的样品，展示了NC、NHC和NHHC转移的应用。将NHHC方法应用于85个氨基酸的蛋白质Crh的（（13）C:（15）N）标记二聚体形式，揭示了微晶状态下各种单体-单体相互作用。
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