Bannerman Douglas D, Paape Max J, Goff Jesse P, Kimura Kayoko, Lippolis John D, Hope Jayne C
Bovine Functional Genomics Laboratory, Beltsville Agricultural Research Center, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
Vet Res. 2004 Nov-Dec;35(6):681-700. doi: 10.1051/vetres:2004040.
Streptococcus uberis and Serratia marcescens are Gram-positive and Gram-negative bacteria, respectively, that induce clinical mastitis. Once initial host barrier systems have been breached by these pathogens, the innate immune system provides the next level of defense against these infectious agents. The innate immune response is characterized by the induction of pro-inflammatory cytokines, as well as increases in other accessory proteins that facilitate host recognition and elimination of the pathogens. The objective of the current study was to characterize the innate immune response during clinical mastitis elicited by these two important, yet less well-studied, Gram-positive and Gram-negative organisms. The pro-inflammatory cytokine response and changes in the levels of the innate immune accessory recognition proteins, soluble CD14 (sCD14) and lipopolysaccharide (LPS)-binding protein (LBP), were studied. Decreased milk output, induction of a febrile response, and increased acute phase synthesis of LBP were all characteristic of the systemic response to intramammary infection with either organism. Infection with either bacteria similarly resulted in increased milk levels of IL-1 beta, IL-8, IL-10, IL-12, IFN-gamma, TNF-alpha, sCD14, LBP, and the complement component, C5a. However, the duration of and/or maximal changes in the increased levels of these inflammatory markers were significantly different for several of the inflammatory parameters assayed. In particular, S. uberis infection was characterized by the sustained elevation of higher milk levels of IL-1 beta, IL-10, IL-12, IFN-gamma, and C5a, relative to S. marcescens infection. Together, these data demonstrate the variability of the innate immune response to two distinct mastitis pathogens.
乳房链球菌和粘质沙雷氏菌分别是革兰氏阳性菌和革兰氏阴性菌，它们会引发临床乳腺炎。一旦这些病原体突破了宿主的初始屏障系统，先天免疫系统就会提供下一层针对这些感染因子的防御。先天免疫反应的特征是促炎细胞因子的诱导，以及其他辅助蛋白的增加，这些蛋白有助于宿主识别和清除病原体。本研究的目的是描述由这两种重要但研究较少的革兰氏阳性菌和革兰氏阴性菌引发的临床乳腺炎期间的先天免疫反应。研究了促炎细胞因子反应以及先天免疫辅助识别蛋白、可溶性CD14（sCD14）和脂多糖（LPS）结合蛋白（LBP）水平的变化。产奶量下降、发热反应的诱导以及LBP急性期合成增加都是对任何一种微生物进行乳房内感染的全身反应的特征。感染任何一种细菌同样会导致牛奶中IL-1β、IL-8、IL-10、IL-12、IFN-γ、TNF-α、sCD14、LBP和补体成分C5a水平升高。然而，对于所检测的几个炎症参数，这些炎症标志物水平升高的持续时间和/或最大变化存在显著差异。特别是，相对于粘质沙雷氏菌感染，乳房链球菌感染的特征是牛奶中IL-1β、IL-10、IL-12、IFN-γ和C5a水平持续升高。总之，这些数据证明了对两种不同乳腺炎病原体的先天免疫反应的变异性。
