Sadat Asmaa, Farag Alshimaa M M, Elhanafi Driss, Awad Amal, Elmahallawy Ehab Kotb, Alsowayeh Noorah, El-Khadragy Manal F, Elshopakey Gehad E
Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Animals (Basel). 2023 Mar 1;13(5):892. doi: 10.3390/ani13050892.
The study aimed to investigate the mastitis' emerging causative agents and their antimicrobial sensitivity, in addition to the hematological, biochemical indicators, oxidative biomarkers, acute phase protein (APP), and inflammatory cytokine changes in dairy farms in Gamasa, Dakahlia Governorate, Egypt. One hundred Holstein Friesian dairy cattle with clinical and subclinical mastitis were investigated and were allocated into three groups based on a thorough clinical examination. and were found responsible for the clinical and subclinical mastitis in dairy farms, respectively. Multiple drug resistance (MDR) was detected in 100%, and 94.74% of and isolates, respectively. Significantly low RBCs count, Hb, and PCV values were detected in mastitic cows compared with both subclinical mastitic and control groups; moreover, WBCs, lymphocytes, and neutrophil counts were significantly diminished in mastitic cows compared to the controls. Significantly higher levels of AST, LDH, total protein, and globulin were noticed in both mastitic and subclinical mastitic cows. The haptoglobin, fibrinogen, amyloid A, ceruloplasmin, TNF-α, IL-1β, and IL-6 levels were statistically increased in mastitic cows compared to the controls. Higher MDA levels and reduction of TAC and catalase were identified in all the mastitic cases compared to the controls. Overall, the findings suggested potential public health hazards due to antimicrobial resistance emergence. Meanwhile, the APP and cytokines, along with antioxidant markers can be used as early indicators of mastitis.
该研究旨在调查埃及达卡利亚省加马萨奶牛场乳腺炎新出现的病原体及其抗菌敏感性，以及血液学、生化指标、氧化生物标志物、急性期蛋白（APP）和炎性细胞因子的变化。对100头患有临床型和亚临床型乳腺炎的荷斯坦弗里生奶牛进行了调查，并根据全面的临床检查将它们分为三组。结果发现，[具体病原体1]和[具体病原体2]分别导致奶牛场的临床型和亚临床型乳腺炎。在[具体病原体1]和[具体病原体2]的分离株中，多重耐药（MDR）检出率分别为100%和94.74%。与亚临床型乳腺炎奶牛组和对照组相比，患乳腺炎奶牛的红细胞计数、血红蛋白和红细胞压积值显著降低；此外，与对照组相比，患乳腺炎奶牛的白细胞、淋巴细胞和中性粒细胞计数显著减少。在患乳腺炎和亚临床型乳腺炎的奶牛中，天冬氨酸转氨酶、乳酸脱氢酶、总蛋白和球蛋白水平均显著升高。与对照组相比，患乳腺炎奶牛的触珠蛋白、纤维蛋白原、淀粉样蛋白A、铜蓝蛋白、肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6水平在统计学上有所增加。与对照组相比，所有患乳腺炎病例的丙二醛水平较高，总抗氧化能力和过氧化氢酶水平降低。总体而言，研究结果表明抗菌药物耐药性的出现可能对公共卫生造成危害。同时，APP和细胞因子以及抗氧化标志物可作为乳腺炎的早期指标。
