Hosokawa Masashi, Kudo Masahiro, Maeda Hayato, Kohno Hiroyuki, Tanaka Takuji, Miyashita Kazuo
Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato, Hakodate, Hakodate, Hokkaido 041-8611, Japan.
Biochim Biophys Acta. 2004 Nov 18;1675(1-3):113-9. doi: 10.1016/j.bbagen.2004.08.012.
The effect of fucoxanthin, from the edible seaweed Undaria pinnatifida on viability of colon cancer cells and induction of apoptosis was investigated. Fucoxanthin remarkably reduced the viability of human colon cancer cell lines, Caco-2, HT-29 and DLD-1. Furthermore, treatment with fucoxanthin induced DNA fragmentation, indicating apoptosis. The DNA fragmentation in Caco-2 cells treated with 22.6 microM fucoxanthin for 24 h was 10-fold higher than in the control. Fucoxanthin suppressed the level of Bcl-2 protein. Also, DNA fragmentation induced by fucoxanthin was partially inhibited by a caspase inhibitor Z-VAD-fmk. Moreover, combined treatment with 3.8 microM fucoxanthin and 10 microM troglitazone, which is a specific ligand for peroxisome proliferator-activated receptor (PPAR) gamma, effectively decreased the viability of Caco-2 cells. However, separate treatments with these same concentrations of fucoxanthin nor troglitazone did not affect cell viability. These findings indicate that fucoxanthin may act as a chemopreventive and/or chemotherapeutic carotenoid in colon cancer cells by modulating cell viability in combination with troglitazone.
研究了来自可食用海藻裙带菜的岩藻黄质对结肠癌细胞活力及凋亡诱导的影响。岩藻黄质显著降低了人结肠癌细胞系Caco-2、HT-29和DLD-1的活力。此外，用岩藻黄质处理可诱导DNA片段化，表明发生了凋亡。用22.6微摩尔岩藻黄质处理Caco-2细胞24小时后，DNA片段化程度比对照高10倍。岩藻黄质抑制了Bcl-2蛋白的水平。此外，岩藻黄质诱导的DNA片段化被半胱天冬酶抑制剂Z-VAD-fmk部分抑制。而且，3.8微摩尔岩藻黄质与10微摩尔曲格列酮（一种过氧化物酶体增殖物激活受体（PPAR）γ的特异性配体）联合处理，有效降低了Caco-2细胞的活力。然而，用相同浓度的岩藻黄质或曲格列酮单独处理并不影响细胞活力。这些发现表明，岩藻黄质可能通过与曲格列酮联合调节细胞活力，在结肠癌细胞中充当化学预防和/或化疗类胡萝卜素。
