Lara-Hernández Gabriel, Ramos-Silva José Alberto, Pérez-Soto Elvia, Figueroa Mario, Flores-Berrios Ericka Patricia, Sánchez-Chapul Laura, Andrade-Cabrera José Luis, Luna-Angulo Alexandra, Landa-Solís Carlos, Avilés-Arnaut Hamlet
Laboratorio de Biomedicina y Salud Ocupacional, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Ciudad de México 07320, Mexico.
Facultad de Ciencias Biológicas, Instituto de Biotecnología, Universidad Autónoma de Nuevo León, Campus Ciudad Universitaria, Av. Universidad S/N, San Nicolás de los Garza 66455, Mexico.
Nutrients. 2024 Dec 11;16(24):4274. doi: 10.3390/nu16244274.
Plants and algae harbor diverse molecules with antioxidant activity and have been demonstrated to directly inhibit cancer cell growth and mitigate the oxidative damage associated with certain antitumor therapies. While antioxidant supplementation, either alone or in combination with chemotherapy, has shown promise in improving quality of life, further research is needed to explore the effects of antioxidant combinations on specific cancer cell lines. In this study, the in vitro cytotoxic and apoptotic properties of natural compounds derived from plants and algae, as well as certain dietary supplements, were investigated against various human cancer cell lines, including bone, leukemia, colorectal, breast, and prostate cancers. Apple polyphenols, fucoxanthin, and plant-derived tocotrienols exhibited cytotoxic effects across all lines; however, tocotrienols demonstrated the most potent, time-dependent cytotoxic activity, with a half-inhibitory concentration (IC) of 4.3 μg/mL in bone cancer cells. Analysis of dietary supplements 2.1, 4.0, and 10.0 revealed that supplement 10.0 exhibited specific cytotoxic activity against bone cancer line TIB-223 and colorectal cancer cell line Caco2, with IC values of 126 μg/mL and 158 μg/mL, respectively. Both tocotrienols and supplement 10.0 induced morphological changes in TIB-223 cells, inhibited cell migration (anti-metastatic activity), and promoted apoptosis, as evidenced by caspase 3/7 activation in both bone and colorectal cancer cells. These findings provide valuable insights for the development of targeted dietary supplements to enhance the anticancer effect of conventional chemotherapy in specific cancer types.
植物和藻类含有多种具有抗氧化活性的分子，并且已被证明能直接抑制癌细胞生长，并减轻与某些抗肿瘤疗法相关的氧化损伤。虽然单独或与化疗联合使用抗氧化剂补充剂在改善生活质量方面已显示出前景，但仍需要进一步研究来探索抗氧化剂组合对特定癌细胞系的影响。在本研究中，研究了源自植物和藻类的天然化合物以及某些膳食补充剂对包括骨癌、白血病、结直肠癌、乳腺癌和前列腺癌在内的各种人类癌细胞系的体外细胞毒性和凋亡特性。苹果多酚、岩藻黄质和植物源生育三烯酚在所有细胞系中均表现出细胞毒性作用；然而，生育三烯酚表现出最有效的、时间依赖性的细胞毒性活性，在骨癌细胞中的半抑制浓度（IC）为4.3μg/mL。对膳食补充剂2.1、4.0和10.0的分析表明，补充剂10.0对骨癌细胞系TIB-223和结直肠癌细胞系Caco2表现出特异性细胞毒性活性，IC值分别为126μg/mL和158μg/mL。生育三烯酚和补充剂10.0均诱导TIB-223细胞发生形态变化，抑制细胞迁移（抗转移活性），并促进凋亡，骨癌细胞和结直肠癌细胞中的半胱天冬酶3/7激活证明了这一点。这些发现为开发靶向膳食补充剂以增强传统化疗在特定癌症类型中的抗癌效果提供了有价值的见解。
