成年大鼠在体内长期接触铅后，其脑髓鞘中2',3'-环核苷酸3'-磷酸二酯酶（CNPase）的活性。

CNPase activity in myelin from adult rat brains after prolonged lead exposure in vivo.

作者信息

Dabrowska-Bouta Beata, Sulkowski Grzegorz, Struzyńska Lidia, Rafałowska Urszula

机构信息

Laboratory of Pathobiochemistry of the Central Nervous System, Department of Neurochemistry, Medical Research Centre, Polish Academy of Sciences, 5 Pawińskiego St., 02-106 Warsaw, Poland.

出版信息

Chem Biol Interact. 2004 Nov 20;150(2):171-8. doi: 10.1016/j.cbi.2004.08.003.

DOI:10.1016/j.cbi.2004.08.003

PMID:15535987

Abstract

The present study investigated the sensitivity of rats cerebral myelin to prolonged toxicity of lead (Pb) that imitates environmental exposure to this metal. The results indicated that 90 days exposure of young adult rats to lead in drinking water affects the morphology of myelin sheaths, expressed in disintegration of its multilamellar structure. Both, the protein content and the activity of the myelin-specific enzyme CNPase (2',3'-cyclic nucleotide 3-phosphodiesterase), were lowered. The Michaelis-Menten kinetic for CNPase in myelin obtained from control and Pb-treated rats was different. Km increased and Vmax decreased when compared to controls. Observed disturbances in enzyme activity may be one of the potential reasons of the ultrastructural changes. It is thus tempting to speculate that Pb may be considered as a one of the factors contributing to demyelinating diseases.

摘要

本研究调查了大鼠脑髓磷脂对模拟环境铅暴露的长期毒性的敏感性。结果表明，成年幼鼠饮用含铅水90天会影响髓鞘形态，表现为其多层结构的解体。髓鞘蛋白含量和髓鞘特异性酶CNPase（2'，3'-环核苷酸3-磷酸二酯酶）的活性均降低。从对照大鼠和铅处理大鼠获得的髓鞘中CNPase的米氏动力学不同。与对照组相比，Km增加而Vmax降低。观察到的酶活性紊乱可能是超微结构变化的潜在原因之一。因此，有人推测铅可能是导致脱髓鞘疾病的因素之一。