Dabrowska-Bouta B, Sulkowski G, Walski M, Struzyńska L, Lenkiewicz A, Rafałowska U
Laboratory of Pathobiochemistry of the Central Nervous System, Medical Research Centre, Polish Academy of Sciences, Warsaw.
Exp Toxicol Pathol. 2000 Jun;52(3):257-63. doi: 10.1016/S0940-2993(00)80043-3.
The aim of the present study was to assess the sensitivity of central nervous system myelin to acute Pb-toxicity in an animal model, that imitates lead toxicity in occupationally exposed workers, or in occasional incidents of poisoning. Our results indicated that in vivo acute lead intoxication affected both the morphology of myelin and enzymatic activity of the myelin marker, CNPase (2'3'-cyclic nucleotide 3'-phosphodiesterase). The multilayered structure of myelin sheaths was regionally disturbed, with loosely arranged membranes or ovoid-shaped swollen fragments. The activity of CNPase was diminished and Michaelis-Menten kinetics showed a decreased affinity and lower velocity of the enzyme. These data suggest that the disturbances in CNPase activity may contribute, in some extent, to the changes in myelin morphology observed in acute Pb-intoxication.
本研究的目的是在一种动物模型中评估中枢神经系统髓鞘对急性铅中毒的敏感性，该模型模拟职业暴露工人或偶发中毒事件中的铅毒性。我们的结果表明，体内急性铅中毒会影响髓鞘的形态以及髓鞘标志物2',3'-环核苷酸3'-磷酸二酯酶（CNPase）的酶活性。髓鞘多层结构出现局部紊乱，膜排列松散或呈卵形肿胀碎片。CNPase的活性降低，米氏动力学显示该酶的亲和力下降且速度降低。这些数据表明，CNPase活性的紊乱可能在一定程度上导致了急性铅中毒时观察到的髓鞘形态变化。
