Tangrea Michael A, Chuaqui Rodrigo F, Gillespie John W, Ahram Mamoun, Gannot Gallya, Wallis Benjamin S, Best Carolyn J M, Linehan W Marston, Liotta Lance A, Pohida Thomas J, Bonner Robert F, Emmert-Buck Michael R
Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.
Diagn Mol Pathol. 2004 Dec;13(4):207-12. doi: 10.1097/01.pdm.0000135964.31459.bb.
Tissue microdissection is an important method for the study of disease states. However, it is difficult to perform high-throughput molecular analysis with current techniques. We describe here a prototype version of a novel technique (expression microdissection) that allows for the procurement of desired cells via molecular targeting. Expression microdissection (xMD) offers significant advantages over available methods, including an increase in dissection speed of several orders of magnitude. xMD may become a valuable tool for investigators studying cancer or other disease states in patient specimens and animal models.
组织显微切割是研究疾病状态的一种重要方法。然而，利用当前技术进行高通量分子分析存在困难。我们在此描述一种新技术（表达显微切割）的原型版本，该技术可通过分子靶向获取所需细胞。与现有方法相比，表达显微切割（xMD）具有显著优势，包括切割速度提高几个数量级。对于在患者标本和动物模型中研究癌症或其他疾病状态的研究人员而言，xMD可能会成为一种有价值的工具。
