Département de Chimie Moléculaire (DCM-UMR CNRS 5250), Institut de Chimie Moléculaire de Grenoble, (ICMG-FR CNRS 2607), Université Joseph Fourier Grenoble, France.
Département de Chimie Moléculaire (DCM-UMR CNRS 5250), Institut de Chimie Moléculaire de Grenoble, (ICMG-FR CNRS 2607), Université Joseph Fourier Grenoble, France ; Centre de Recherche sur les Macromolécules Végétales (CERMAV-UPR CNRS 5301), Institut de Chimie Moléculaire de Grenoble, (ICMG-FR CNRS 2607) Grenoble, France.
Front Chem. 2013 Jul 12;1:10. doi: 10.3389/fchem.2013.00010. eCollection 2013.
This paper reports on the impedimetric transduction of binding reaction between polymerized saccharides and target lectins. The controlled potential electro-oxidation of pyrrole-lactosyl and pyrrole-3'-sialyllactosyl at 0.95 V vs. Ag/AgCl, provides thin and reproducible poly(pyrrole-saccharide) films. The affinity binding of two lectins: Arachis hypogaea, (PNA) and Maackia amurensis (MAA) onto poly(pyrrole-lactosyl) and poly(pyrrole-3'-sialyllactosyl) electrodes, was demonstrated by cyclic voltammetry in presence of ruthenium hexamine and hydroquinone. In addition, rotating disk experiments were carried out to determine the permeability of both polypyrrole films and its evolution after incubating with lectin target. Finally, the possibility of using the poly(pyrrole-lactosyl) or poly(pyrrole-3'-siallyllactosyl) films for the impedimetric transduction of the lectin binding reaction, was investigated with hydroquinone (2 × 10(-3) mol L(-1)) as a redox probe in phosphate buffer. The resulting impedance spectra were interpreted and modeled as an equivalent circuit indicating that charge transfer resistance (R ct) and relaxation frequency (f°) parameters are sensitive to the lectin binding. R ct increases from 77 to 97 Ω cm(2) for PNA binding and from 93 to 131 Ω cm(2) for MAA binding. In parallel, f° decreases from 276 to 222 Hz for PNA binding and from 223 to 131 Hz for MAA binding. This evolution of both parameters reflects the steric hindrances generated by the immobilized lectins towards the permeation of the redox probe.
本文报告了聚合物糖与靶凝集素之间结合反应的阻抗转换。在 0.95 V 对 Ag/AgCl 下，吡咯乳糖基和吡咯-3'-唾液乳糖基的受控电位电氧化提供了薄且可重复的聚（吡咯-糖）膜。两种凝集素：花生（PNA）和马卡西亚（MAA）在聚（吡咯-乳糖基）和聚（吡咯-3'-唾液乳糖基）电极上的亲和结合，通过在存在钌六胺和对苯二酚的情况下进行循环伏安法得到证明。此外，进行了旋转圆盘实验以确定两种聚吡咯膜的渗透性及其与靶标凝集素孵育后的变化。最后，研究了使用聚（吡咯-乳糖基）或聚（吡咯-3'-唾液乳糖基）膜作为电化学传感器，以对苯二酚（2×10（-3）mol L（-1））作为氧化还原探针，在磷酸盐缓冲液中进行的凝集素结合反应的阻抗转换的可能性。解释和模拟了所得阻抗谱作为等效电路，表明电荷转移电阻（Rct）和弛豫频率（f°）参数对凝集素结合敏感。对于 PNA 结合，Rct 从 77 增加到 97 Ω cm（2），对于 MAA 结合，Rct 从 93 增加到 131 Ω cm（2）。同时，对于 PNA 结合，f°从 276 降低到 222 Hz，对于 MAA 结合，f°从 223 降低到 131 Hz。这两个参数的变化反映了固定化凝集素对氧化还原探针渗透产生的空间位阻。
