一种用于空间控制构建安培型生物传感器的生物素化导电聚吡咯。

A biotinylated conducting polypyrrole for the spatially controlled construction of an amperometric biosensor.

作者信息

Cosnier S, Stoytcheva M, Senillou A, Perrot H, Furriel R P, Leone F A

机构信息

Laboratoire d'Electrochimie Organique et de Photochimie Redox, UMR CNRS 5630, Université Joseph Fourier, Grenoble, France.

出版信息

Anal Chem. 1999 Sep 1;71(17):3692-7. doi: 10.1021/ac9901788.

DOI:10.1021/ac9901788

PMID:10489522

Abstract

A new biotin derivative functionalized by an electropolymerizable pyrrole group has been synthesized. The electrooxidation of this biotin pyrrole has allowed the formation of biotinylated conducting polypyrrole films in organic electrolyte. Gravimetric measurements based on a quartz crystal microbalance, modified by the biotinylated polymer, revealed an avidin-biotin-specific binding at the interface of polymer-solution. The estimated mass increase corresponded to the anchoring of 1.5 avidin monolayers on the polypyrrole surface. In addition, the subsequent grafting of biotinylated glucose oxidase was corroborated by electrochemical permeation studies. Enzyme multilayers composed of glucose oxidase or polyphenol oxidase were elaborated on the electrode surface modified by the biotinylated polypyrrole film. The amperometric response of the resulting biosensors to glucose or catechol has been studied at +0.6 or -0.2 V vs SCE, respectively.

摘要

一种通过可电聚合的吡咯基团功能化的新型生物素衍生物已被合成。这种生物素吡咯的电氧化使得在有机电解质中形成生物素化的导电聚吡咯膜成为可能。基于经生物素化聚合物修饰的石英晶体微天平的重量测量，揭示了在聚合物 - 溶液界面处抗生物素蛋白 - 生物素特异性结合。估计的质量增加对应于1.5个抗生物素蛋白单分子层在聚吡咯表面的锚定。此外，生物素化葡萄糖氧化酶的后续接枝通过电化学渗透研究得到证实。由葡萄糖氧化酶或多酚氧化酶组成的酶多层膜在经生物素化聚吡咯膜修饰的电极表面上得以构建。所得生物传感器对葡萄糖或邻苯二酚的安培响应分别在相对于饱和甘汞电极（SCE）为 +0.6 或 -0.2 V 下进行了研究。