Pillai M G, Thampi B S, Menon V P, Leelamma S
Department of Biochemistry, University of Kerala, Kariavattom, Trivandurm, Kerala, India.
J Nutr Biochem. 1999 Sep;10(9):555-60. doi: 10.1016/s0955-2863(99)00035-2.
The influence of dietary fiber from coconut kernel isolated by the neutral detergent fiber method on the antioxidant status in rats treated with the colon specific carcinogen 1,2-dimethylhydrazine (DMH) was studied in rats fed a high-fat diet for 15 weeks. The DMH-treated fiber group showed higher levels of lipid peroxides than the control group treated with DMH at the preneoplastic and neoplastic stages. Free fatty acid levels were found to decrease significantly in the DMH-treated control group, whereas it was near normal in the fiber groups. Superoxide dismutase and catalase activity also were found to be increased in the liver, intestine, proximal colon, and distal colon. Glutathione levels in all the tissues studied showed significant decreases in the fiber group. The results suggest that coconut kernel fiber can protect cells from loss of oxidative capacity with the administration of the procarcinogen DMH.
采用中性洗涤剂纤维法从椰仁中分离出膳食纤维，研究其对经结肠特异性致癌物1,2 - 二甲基肼（DMH）处理的大鼠抗氧化状态的影响。将大鼠喂饲高脂饮食15周，在癌前和肿瘤形成阶段，DMH处理的纤维组脂质过氧化物水平高于DMH处理的对照组。DMH处理的对照组游离脂肪酸水平显著降低，而纤维组接近正常水平。超氧化物歧化酶和过氧化氢酶活性在肝脏、肠道、近端结肠和远端结肠中也有所增加。在所有研究组织中，纤维组的谷胱甘肽水平显著降低。结果表明，椰仁纤维可在给予致癌物前体DMH时保护细胞免受氧化能力丧失的影响。
