Gosselin Robert C, Janatpour Kim, Larkin Edward C, Lee Yanlap P, Owings John T
Department of Pathology, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Am J Clin Pathol. 2004 Dec;122(6):843-8. doi: 10.1309/7V2K-P1HP-9Q29-BMN2.
We sought to compare coagulation test results obtained from patients using 2 plastic blood collection tubes and the traditional glass blood collection tube. Blood specimens were obtained from 241 patients in 3.2% buffered sodium citrate using standard glass tubes, in 3.2% buffered sodium citrate in plastic tubes, and in 3.2% sodium citrate "sandwich" tubes (plastic within plastic). All samples were obtained and processed contemporaneously and tested for prothrombin time (PT) and activated partial thromboplastin time (aPTT). Residual plasma was frozen at -70 degrees C for future testing, including fibrinogen, antithrombin, plasminogen, protein C and protein S (functional and antigenic), dilute Russell viper venom time (DRVVT), ristocetin cofactor, factor XIII, D dimer, anti-Xa activity, and prothrombin fragment. Although paired t test analysis revealed statistically significant differences (P < .05) between glass and plastic for PT, aPTT, fibrinogen, protein C (functional and antigenic), functional protein S, DRVVT and confirmation method, antithrombin, and factor XIII, these differences were not considered clinically significant.
我们试图比较使用两种塑料采血管和传统玻璃采血管的患者的凝血试验结果。使用标准玻璃管、塑料管中含3.2%枸橼酸钠缓冲液的采血管以及“三明治”塑料管（两层塑料中间夹着枸橼酸钠缓冲液）从241名患者采集血标本。所有样本均同时采集和处理，并检测凝血酶原时间（PT）和活化部分凝血活酶时间（aPTT）。剩余血浆在-70℃冷冻以备将来检测，包括纤维蛋白原、抗凝血酶、纤溶酶原、蛋白C和蛋白S（功能和抗原）、稀释蝰蛇毒时间（DRVVT）、瑞斯托菌素辅因子、因子 XIII、D-二聚体、抗Xa活性和凝血酶原片段。尽管配对t检验分析显示玻璃管和塑料管在PT、aPTT、纤维蛋白原、蛋白C（功能和抗原）、功能性蛋白S、DRVVT及确认方法、抗凝血酶和因子 XIII方面存在统计学显著差异（P < 0.05），但这些差异在临床上不被认为具有显著意义。
