Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna Pot 113, SI1000 Ljubljana, Slovenia.
Molecules. 2023 Jan 4;28(2):486. doi: 10.3390/molecules28020486.
Citrate anticoagulant concentration affects the results of coagulation tests. Until now, the end user had no direct insight into the quality of evacuated blood collection tubes. By introducing an easy-to-perform UV spectrometric method for citrate determination on a purified water model, we enabled the evaluation of (1) the accuracy of the anticoagulant amount added into the tubes by a producer, (2) the accuracy of the volume of anticoagulant solution in the tube at the instant of examination, (3) the anticoagulant concentrations at a draw volume. We examined the Vacuette, Greiner BIO-ONE, Vacutube, LT Burnik d.o.o., and BD Vacutainer tubes. The anticoagulant amount added into the tubes during production had a relative bias between 3.2 and 23.0%. The anticoagulant volume deficiency at the instant of examination expressed as a relative bias ranged between -11.6 and -91.1%. The anticoagulant concentration relative bias after the addition of purified water in a volume that equalled a nominal draw volume extended from 9.3 to 25.7%. Draw-volume was mostly compliant during shelf life. Only Vacutube lost water over time. Contamination with potassium, magnesium, or both was observed in all the tubes but did not exceed a 0.21 mmol/L level. This study enables medical laboratories to gain insight into the characteristics of the citrate blood collection tubes as one of the preanalytical variables. In situations that require anticoagulant adjustment for accurate results, this can help make the right decisions. The methodology gives producers additional means of controlling the quality of their production process.
柠檬酸盐抗凝剂浓度会影响凝血测试的结果。直到现在，终端用户都无法直接了解真空采血管的质量。通过引入一种易于执行的 UV 分光光度法，我们可以在纯化水模型上测定柠檬酸盐，从而评估（1）生产商添加到管中的抗凝剂的量的准确性，（2）检查时管内抗凝剂溶液的体积的准确性，（3）抽取体积时的抗凝剂浓度。我们检查了 Vacuette、Greiner BIO-ONE、Vacutube、LT Burnik d.o.o. 和 BD Vacutainer 管。生产过程中添加到管中的抗凝剂的量的相对偏差在 3.2%至 23.0%之间。检查时抗凝剂体积不足的相对偏差在-11.6%至-91.1%之间。添加与标称抽取体积相等的纯化水后抗凝剂浓度的相对偏差从 9.3%延伸到 25.7%。在保质期内，抽取体积大多符合要求。只有 Vacutube 随着时间的推移失去了水分。所有管中都观察到钾、镁或两者的污染，但都没有超过 0.21mmol/L 水平。本研究使医学实验室能够深入了解柠檬酸盐血液采集管作为分析前变量之一的特性。在需要抗凝剂调整以获得准确结果的情况下，这有助于做出正确的决策。该方法为生产商提供了额外的手段来控制其生产过程的质量。
