用7,12-二甲基苯并(a)蒽诱导大鼠实验性乳腺癌发生
Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene.
作者信息
Barros Alfredo Carlos S D, Muranaka Elisa Naomi K, Mori Lincon Jo, Pelizon Christina Helena T, Iriya Kyoshi, Giocondo Giovana, Pinotti José Aristodemo
机构信息
Discipline of Gynecology, Hospital das Clínicas, Faculty of Medicine, University of São Paulo - São Paulo/SP, Brazil.
出版信息
Rev Hosp Clin Fac Med Sao Paulo. 2004 Oct;59(5):257-61. doi: 10.1590/s0041-87812004000500006. Epub 2004 Oct 29.
PURPOSE
To test an experimental model of chemical mammary carcinogenesis induction in rats.
METHODS
Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy.
RESULTS
Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis.
CONCLUSION
This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.
目的
测试大鼠化学性乳腺癌发生诱导的实验模型。
方法
20只47日龄的年轻未孕斯普拉格-道利雌性大鼠经口灌胃给予20毫克7,12-二甲基苯并(a)蒽（DMBA）。之后，在第8周和第13周检查它们的乳腺。实验结束时，处死动物，测量并称重乳腺肿瘤。使用光学显微镜分析肿瘤碎片。
结果
注射DMBA后8周，16只大鼠出现至少1个乳腺肿瘤（80%）。13周后，所有大鼠（100%）均发生乳腺癌，经组织病理学分析证实。
结论
这种化学性乳腺诱导致癌的实验动物模型是可行的，可用于进一步研究致瘤生物调节物质的作用。
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