Dreher Matthew R, Elas Martyna, Ichikawa Kazuhiro, Barth Eugene D, Chilkoti Ashutosh, Rosen Gerald M, Halpern Howard J, Dewhirst Mark
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
Med Phys. 2004 Oct;31(10):2755-62. doi: 10.1118/1.1782677.
Hyperthermia, as an adjuvant with radiation and chemotherapy, has shown promise in the treatment of cancer. The relevant biological effects of a hyperthermia treatment are both time and temperature-dependent, creating a need for accurate thermometry. We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. When examined with electron paramagnetic resonance (EPR) spectroscopy, the ELP-nitroxide conjugate has temperature-dependent spectral line widths whose predictive accuracy is approximately 0.3 degrees C (80 microM). We believe that the temperature-dependent changes observed in the EPR spectrum are due to the combined effect of temperature, viscosity and effective radius on the rotational correlation time of the ELP-nitroxide conjugate.
热疗作为放疗和化疗的辅助手段，已在癌症治疗中显示出前景。热疗的相关生物学效应既与时间有关，也与温度有关，因此需要精确的温度测量。我们提出了一种新型的非侵入性温度测量方法，该方法将温度响应性生物聚合物弹性蛋白样多肽（ELP）与氮氧化物结合，以产生ELP-氮氧化物共轭物。当用电子顺磁共振（EPR）光谱检查时，ELP-氮氧化物共轭物具有与温度相关的谱线宽度，其预测精度约为0.3摄氏度（80微摩尔）。我们认为，在EPR光谱中观察到的与温度相关的变化是由于温度、粘度和有效半径对ELP-氮氧化物共轭物旋转相关时间的综合影响。
