Department of Gastrointestinal Surgery, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
Department of Gastrointestinal Surgery, Nippon Medical School Chiba Hokusoh Hospital, Chiba, Japan.
Sci Rep. 2022 Mar 21;12(1):4811. doi: 10.1038/s41598-022-08962-y.
Genetic analysis and culturing techniques for gastric non-Helicobacter pylori Helicobacter (NHPH) are progressing. NHPH is reported to accompany nodular gastritis, gastric MALT lymphoma, and mild gastritis. However, only a few gastric cancer cases infected by NHPH have been reported. PCR analysis specific for NHPH and H. pylori was performed for DNA from gastric mucosa of 282 Korean gastric cancer patients, who were treated with endoscopic submucosal dissection. For more precise strain detection of NHPH, NHPH-positive mucosa was stained by immunohistochemistry specific for Helicobacter suis. The Cancer Genome Atlas (TCGA) classification was analyzed for these 3 gastric cancer sub-groups by in situ hybridization and immunohistochemistry. Among 281 patients, 3 patients (1.1%) were positive for NHPH. One patient (Patient 1) was also positive for H. pylori by PCR, another patient (Patient 3) was positive for serum IgG for H. pylori, and the other patient (Patient 2) had no evidence for H. pylori infection. Gastric mucosa of Patients 2 and 3 were positive for H. suis staining. All three NHPH-positive gastric cancers were located in the antrum, and belonged to the Chromosomal Instability Type of TCGA classification. Gastric NHPH can be a cause of gastric cancer, although likely with lower pathogenesis than H. pylori.
正在进行非幽门螺杆菌胃幽门螺旋菌（NHPH）的基因分析和培养技术研究。据报道，NHPH 伴随着结节性胃炎、胃黏膜相关淋巴组织淋巴瘤和轻度胃炎。然而，只有少数感染 NHPH 的胃癌病例被报道。对 282 例接受内镜黏膜下剥离术治疗的韩国胃癌患者的胃黏膜 DNA 进行了针对 NHPH 和幽门螺杆菌的 PCR 分析。为了更精确地检测 NHPH 的菌株，对 NHPH 阳性黏膜进行了针对猪幽门螺旋菌的免疫组织化学染色。通过原位杂交和免疫组织化学分析了这 3 个胃癌亚组的癌症基因组图谱（TCGA）分类。在 281 例患者中，3 例（1.1%）患者 NHPH 阳性。1 例患者（患者 1）PCR 也检测到幽门螺杆菌阳性，另 1 例患者（患者 3）血清 IgG 对幽门螺杆菌阳性，另 1 例患者（患者 2）无幽门螺杆菌感染证据。患者 2 和 3 的胃黏膜对 H. suis 染色呈阳性。所有 3 例 NHPH 阳性胃癌均位于胃窦，属于 TCGA 分类的染色体不稳定型。尽管 NHPH 可能比幽门螺杆菌的发病机制更低，但它可能是胃癌的一个原因。
