苹果酸酶是集胞藻PCC 6803株在持续光照下实现最佳光合自养生长所必需的，但在昼夜光照条件下则并非必需。
The malic enzyme is required for optimal photoautotrophic growth of Synechocystis sp. strain PCC 6803 under continuous light but not under a diurnal light regimen.
作者信息
Bricker Terry M, Zhang Shulu, Laborde Susan M, Mayer Paul R, Frankel Laurie K, Moroney James V
机构信息
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
出版信息
J Bacteriol. 2004 Dec;186(23):8144-8. doi: 10.1128/JB.186.23.8144-8148.2004.
Abstract
A mutation was recovered in the slr0721 gene, which encodes the decarboxylating NADP(+)-dependent malic enzyme in the cyanobacterium Synechocystis sp. strain PCC 6803, yielding the mutant 3WEZ. Under continuous light, 3WEZ exhibits poor photoautotrophic growth while growing photoheterotrophically on glucose at rates nearly indistinguishable from wild-type rates. Interestingly, under diurnal light conditions (12 h of light and 12 h of dark), normal photoautotrophic growth of the mutant is completely restored.
摘要
在集胞藻PCC 6803中，编码脱羧NADP(+)依赖性苹果酸酶的slr0721基因发生了突变，产生了突变体3WEZ。在持续光照下，3WEZ的光合自养生长较差，而在葡萄糖上进行光合异养生长时，其生长速率与野生型几乎没有区别。有趣的是，在昼夜光照条件下（12小时光照和12小时黑暗），突变体的正常光合自养生长完全恢复。
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