Kapur Payal, Rakheja Dinesh, Gomez Ana M, Sheffield Jeanne, Sanchez Pablo, Rogers Beverly Barton
Department of Pathology, The University of Texas Southwestern Medical School, Dallas, TX, USA.
Pediatr Dev Pathol. 2004 Sep-Oct;7(5):453-8; discussion 421. doi: 10.1007/s10024-004-2124-3. Epub 2004 Jul 30.
Chronic villitis is a histologic diagnosis that may be either associated with infection, or termed villitis of undetermined etiology (VUE). The lymphocytic infiltrate in VUE has been reported to consist of maternal lymphocytes, but the origin of the lymphocytic infiltrate in infectious villitis has not been identified. The purpose of our study was to compare the maternal vs. fetal origin of the infiltrating lymphocytes in VUE and syphilitic villitis, and to expand the immunophenotypic data provided by previous studies. Paraffin-embedded placentas from four males with VUE and two males with syphilitic villitis were subjected to fluorescence in situ hybridization (FISH) for the X and Y chromosomes. Serial sections were stained with antibodies to CD3, CD4, CD8, CD68, HLA-DR, and CD20. Quantitation of the relative number of cells marking with each antibody was done for four villi in each slide. CD3 lymphocytes predominated in both VUE and syphilitic villitis, with slightly more CD8 cells compared to CD4 cells. CD68 and HLA-DR positive cells were as frequent as CD3 cells, and B-lymphocytes were rare. Maternal cells were the predominant intravillous population in both VUE and syphilitic villitis, and neutrophils in syphilitic villitis were also maternal. These data indicate that the immune response in both syphilitic villitis and VUE is similar, raising the possibility of a similar immunopathogenetic pathway.
慢性绒毛炎是一种组织学诊断，可能与感染有关，或被称为病因不明的绒毛炎（VUE）。据报道，VUE中的淋巴细胞浸润由母体淋巴细胞组成，但感染性绒毛炎中淋巴细胞浸润的起源尚未明确。我们研究的目的是比较VUE和梅毒绒毛炎中浸润淋巴细胞的母体来源与胎儿来源，并扩展先前研究提供的免疫表型数据。对4例VUE男性和2例梅毒绒毛炎男性的石蜡包埋胎盘进行X和Y染色体的荧光原位杂交（FISH）。连续切片用抗CD3、CD4、CD8、CD68、HLA-DR和CD20抗体染色。对每张载玻片上的四个绒毛进行每种抗体标记细胞相对数量的定量分析。在VUE和梅毒绒毛炎中，CD3淋巴细胞均占主导，与CD4细胞相比，CD8细胞略多。CD68和HLA-DR阳性细胞与CD3细胞一样常见，B淋巴细胞罕见。在VUE和梅毒绒毛炎中，母体细胞均为绒毛内的主要细胞群，梅毒绒毛炎中的中性粒细胞也是母体来源。这些数据表明，梅毒绒毛炎和VUE中的免疫反应相似，增加了存在相似免疫发病机制途径的可能性。
