Pokorski Jonathan K, Witschi Mark A, Purnell Bethany L, Appella Daniel H
Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
J Am Chem Soc. 2004 Nov 24;126(46):15067-73. doi: 10.1021/ja046280q.
Replacing the ethylenediamine portion of aminoethylglycine peptide nucleic acids (aegPNAs) with one or more (S,S)-trans-cyclopentane diamine units significantly increases binding affinity and sequence specificity to complementary DNA, making these modified PNAs ideal for use as nucleic acid probes in genomic analysis. The synthesis and study of this new class of PNAs (tcypPNAs) is described in which trans-cyclopentane diamine has been incorporated into several positions, and in varying number, within PNA backbones of mixed-base sequences.
用一个或多个（S,S）-反式环戊烷二胺单元取代氨乙基甘氨酸肽核酸（aegPNA）的乙二胺部分，可显著提高其与互补DNA的结合亲和力和序列特异性，使这些修饰的PNA成为基因组分析中核酸探针的理想选择。本文描述了这类新型PNA（tcypPNA）的合成与研究，其中反式环戊烷二胺已被引入混合碱基序列的PNA主链中的几个位置，且数量不同。
