Witschi Mark A, Pokorski Jonathan K, Appella Daniel H
Laboratory of Bioorganic Chemistry, NIDDK, NIH, DHHS, Bethesda, Maryland, USA.
Methods Enzymol. 2006;410:189-200. doi: 10.1016/S0076-6879(06)10009-9.
Procedures to attach trans-cyclopentane-modified peptide nucleic acid oligomers to a glass slide are described. Peptide nucleic acids can offer distinct advantages to DNA detection compared to typically used DNA microarrays, especially with regard to chemical stability and quality of data. The trans-cyclopentane modification incorporated into the peptide nucleic acid is, in some cases, essential for successful DNA detection. It is hoped that these peptide nucleic acid-bearing glass slides will find application in several areas of DNA microarray technology.
描述了将反式环戊烷修饰的肽核酸寡聚物连接到载玻片上的方法。与通常使用的DNA微阵列相比，肽核酸在DNA检测方面具有明显优势，特别是在化学稳定性和数据质量方面。在某些情况下，掺入肽核酸中的反式环戊烷修饰对于成功进行DNA检测至关重要。希望这些带有肽核酸的载玻片能在DNA微阵列技术的多个领域得到应用。
