Suyama Eigo, Kawasaki Hiroaki, Wadhwa Renu, Taira Kazunari
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.
J Muscle Res Cell Motil. 2004;25(4-5):303-8. doi: 10.1007/s10974-004-4343-7.
Metastatic tumor cells can migrate from one place to another in the body. This involves their adherence to host cell layers and subsequent transcellular movements by a complex process, molecular basis of which are yet to be clarified. Elucidation of genes functionally involved in metastasis may lead to deeper understanding of the mechanism of cell migration, and identification and designing of metastasis-modulating strategies for cancer therapeutics. We review here cell migration in tumor metastasis and the use of small RNA-based approaches to identify functional genes. We then describe our promising novel approach that uses randomized ribozyme libraries for identification of genes involved in cell migration, a consistent feature of metastatic cells.
转移性肿瘤细胞能够在体内从一个部位迁移至另一个部位。这涉及它们对宿主细胞层的黏附以及随后通过一个复杂过程进行的穿细胞运动，而其分子基础尚待阐明。阐明与转移功能相关的基因可能会加深对细胞迁移机制的理解，并为癌症治疗识别和设计转移调节策略。我们在此综述肿瘤转移中的细胞迁移以及基于小RNA的方法在鉴定功能基因方面的应用。然后我们描述了一种有前景的新方法，该方法利用随机核酶文库来鉴定参与细胞迁移的基因，而细胞迁移是转移性细胞的一个共同特征。
