Bonde Jesper, Hess David A, Nolta Jan A
Department of Internal Medicine, Division of Oncology, Hematopoietic Development and Malignancy Program, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Curr Opin Hematol. 2004 Nov;11(6):392-8. doi: 10.1097/01.moh.0000145672.42503.70.
Exciting advances have been made in the field of hematopoietic stem cell biology during the past year. This review summarizes recent progress in the identification, culture, and in vivo tracking of hematopoietic stem cells.
The roles of Wnt and Notch proteins in regulating stem cell renewal in the microenvironment, and how these molecules can be exploited in ex vivo stem cell culture, are reviewed. The importance of identification of stem cells using functional as well as phenotypic markers is discussed. The novel field of nanotechnology is then discussed in the context of stem cell tracking in vivo. This review concludes with a section on the unexpected potential of bone marrow-derived stem cells to contribute to the repair of damaged tissues. The contribution of cell fusion to explain the latter phenomenon is discussed.
Because of exciting discoveries made recently in the field of stem cell biology, researchers now have improved tools to define novel populations of stem cells, examine them ex vivo using conditions that promote self-renewal, track them into recipients, and determine whether they can contribute to the repair of damaged tissues. These discoveries will significantly advance the field of stem cell transplantation.
过去一年，造血干细胞生物学领域取得了令人振奋的进展。本综述总结了造血干细胞鉴定、培养及体内追踪方面的最新进展。
回顾了Wnt和Notch蛋白在调节微环境中干细胞更新方面的作用，以及如何在体外干细胞培养中利用这些分子。讨论了使用功能和表型标记物鉴定干细胞的重要性。然后在体内干细胞追踪的背景下讨论了纳米技术这一新兴领域。本综述最后一部分论述了骨髓来源干细胞在受损组织修复中发挥作用的意外潜能，并讨论了细胞融合对解释后一现象的作用。
由于干细胞生物学领域最近的激动人心的发现，研究人员现在有了更好的工具来定义新的干细胞群体，在促进自我更新的条件下进行体外研究，追踪它们进入受体体内的情况，并确定它们是否有助于受损组织的修复。这些发现将显著推动干细胞移植领域的发展。
