Chen Ching-Hsein, Liu Tsan-Zon, Kuo Tsun-Cheng, Lu Fung-Jou, Chen Yu-Chin, Chang-Chien Yi-Wen, Lin Chun-Ching
Department of Medical Technology, Fooyin University, Ta-Liao, Kaohsiung Hsien, Taiwan, ROC.
Planta Med. 2004 Nov;70(11):1022-6. doi: 10.1055/s-2004-832641.
Casuarinin has been shown to be an antioxidant in acellular experiments. This study was designed to assess the ability of casuarinin, extracted from Terminalia arjuna, to protect cultured Madin-Darby canine kidney (MDCK) cells against H2O2-mediated oxidative stress. A comparison with trolox, a hydrosoluble vitamin E analogue was performed. MDCK cells were pretreated with casuarinin or trolox for 1 h, then exposed to H2O2. After incubation with 0.8 mM H2O2 for 1 h, casuarinin caused a decrease in intracellular peroxide production as shown by dichlorofluorescein (DCF) fluorescence in a concentration-dependent manner. After 3 h exposure to 8 mM H2O2, the percentage of intracellular glutathione (GSH)-negative cells was reduced in the casuarinin-treated group. Addition of 32mM H2O2 to MDCK cells for 3 h induced an increase in the percentage of cells containing 8-oxoguanine but the level of such cells declined in casuarinin-treated cells. These results show that casuarinin is more effective against H2O2-induced oxidative damage than trolox. The data suggest that casuarinin attenuates H2O2-induced oxidative stress, decreases DNA oxidative damage and prevents the depletion of intracellular GSH in MDCK cells.
在无细胞实验中，儿茶素已被证明是一种抗氧化剂。本研究旨在评估从诃子中提取的儿茶素保护培养的Madin-Darby犬肾（MDCK）细胞免受H2O2介导的氧化应激的能力。并与水溶性维生素E类似物托可索仑进行了比较。MDCK细胞先用儿茶素或托可索仑预处理1小时，然后暴露于H2O2中。在用0.8 mM H2O2孵育1小时后，如二氯荧光素（DCF）荧光所示，儿茶素导致细胞内过氧化物产生以浓度依赖性方式减少。在暴露于8 mM H2O2 3小时后，儿茶素处理组中细胞内谷胱甘肽（GSH）阴性细胞的百分比降低。向MDCK细胞中添加32 mM H2O2 3小时会导致含8-氧代鸟嘌呤的细胞百分比增加，但在儿茶素处理的细胞中此类细胞的水平下降。这些结果表明，儿茶素在对抗H2O2诱导的氧化损伤方面比托可索仑更有效。数据表明，儿茶素可减轻H2O2诱导的氧化应激，减少DNA氧化损伤，并防止MDCK细胞中细胞内GSH的消耗。
