Kuo Po-Lin, Hsu Ya-Ling, Lin Ta-Chen, Lin Liang-Tzung, Chang Jiunn-Kae, Lin Chun-Ching
Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
Planta Med. 2005 Mar;71(3):237-43. doi: 10.1055/s-2005-837823.
Casuarinin, a hydrolyzable tannin isolated from the bark of Terminalia arjuna L. (Combretaceae), was investigated for its antiproliferative activity in human breast adenocarcinoma MCF-7 cells. The results showed that casuarinin inhibited the proliferation of MCF-7 by blocking cell cycle progression in the G0/G1 phase and inducing apoptosis. An enzyme-linked immunosorbent assay showed that casuarinin increased the expression of p21/WAF1 concomitantly as the MCF-7 cells underwent G0/G1 arrest. An enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), might be responsible for the apoptotic effect induced by casuarinin. Our study reports here for the first time that the induction of p21/WAF1 and the activity of Fas/Fas ligand apoptotic system may participate in the antiproliferative activity of casuarinin in MCF-7 cells.
从诃子（使君子科）树皮中分离得到的可水解单宁格木鞣质，对其在人乳腺癌MCF-7细胞中的抗增殖活性进行了研究。结果表明，格木鞣质通过阻断细胞周期在G0/G1期的进程并诱导凋亡来抑制MCF-7细胞的增殖。酶联免疫吸附测定表明，随着MCF-7细胞进入G0/G1期停滞，格木鞣质同时增加了p21/WAF1的表达。Fas/APO-1及其两种配体形式，即膜结合型Fas配体（mFasL）和可溶性Fas配体（sFasL）的增强，可能是格木鞣质诱导凋亡作用的原因。我们的研究首次报道，p21/WAF1的诱导以及Fas/Fas配体凋亡系统的活性可能参与了格木鞣质在MCF-7细胞中的抗增殖活性。
