Panova N G, Shcheveleva E V, Alekseev K S, Mukhortov V G, Zuev A N, Mikhaĭlov S N, Esipov R S, Chuvikovskiĭ D V, Miroshnikov A I
Mol Biol (Mosk). 2004 Sep-Oct;38(5):907-13.
4-Thiouridine and 4-thiothymidine were developed as efficient substrates for spectrophotometric determination of uridine phosphorylase and thymidine phosphorylase activity. 4-Thiouridine has maximum absorbance at 330 nm (pH 7.5). The change in extinction coefficient for 4-thiouridine/4-thiouracil, deltaepsilon is 3000 M(-1) x cm(-1). It appeared that 4-thiouridine is a good substrate for uridine phosphorylase with Michaelis-Menten constant 130 microM and kcat 49 s(-1). In the case of 4-thiothymidine/4-thiothymine deltaepsilon is even larger: 5000 M(-1) x cm(-1) at 336 nm.
