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[采用切向流超滤法测定水中病毒浓度。实验条件下的回收效率]

[The concentration of viruses in water using the tangential flow ultrafiltration. Recovery effectiveness in experimental conditions].

作者信息

Bigliardi L, Cesari C, Zoni R, Sansebastiano G E

机构信息

Dipartimento di Sanità Pubblica, Sez. di Igiene, Università degli Studi di Parma.

出版信息

Ann Ig. 2004 Jan-Apr;16(1-2):281-9.

Abstract

Poliovirus 1 concentration tests were carried out in artificially contaminated water by tangential flow ultrafiltration with Polisulfone filters 100000 MWCO. The tests were performed in 1 and in 20 liters of waters. The filters were conditioned and eluted respectively with Beef extract 3% and with glicina 1% at pH 7 and pH 9. The recovery mean using Beef extract resulted properly good, about the 83% and comparable to percentages we obtained in previous works with filters in cellulose nitrate and Virosorb filters. The viral recovery was low using the glicina for conditioning and eluting the filters.

摘要

使用截留分子量为100000的聚砜过滤器,通过切向流超滤在人工污染的水中进行脊髓灰质炎病毒1型浓度测试。测试在1升和20升水中进行。分别用3%牛肉提取物以及pH值为7和pH值为9的1%甘氨酸对过滤器进行预处理和洗脱。使用牛肉提取物的回收率相当不错,约为83%,与我们之前使用硝酸纤维素过滤器和Virosorb过滤器的工作中获得的百分比相当。使用甘氨酸对过滤器进行预处理和洗脱时,病毒回收率较低。

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