Bagordo Francesco, Grassi Tiziana, Idolo Adele, Serio Francesca, Gabutti Giovanni, De Donno Antonella
Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Prov.le Lecce-Monteroni, Lecce, 73100, Italy.
Food Environ Virol. 2013 Jul 30. doi: 10.1007/s12560-013-9119-z.
The purpose of this study was to evaluate "in field" the accumulation of virus in shellfish and compare it with the concentration of bacterial indicators. Individuals of Mytilus galloprovincialis were placed in two sampling station located in a contaminated coastal bay and in one control station located one kilometer offshore. The presence of Rotavirus and E. coli was assessed weekly both in seawater and in shellfish samples. The Rotavirus genome was detected in water, preliminarily concentrated by tangential flow ultrafiltration method, and in hepatopancreas of mussels by Real-Time PCR. E. coli was enumerated in water matrices by a filtering method and in mussels by the MPN method. Rotaviruses were not recorded in seawater, while in mussels they were detected since third week after placement. E. coli in mussels were always below the limits set in the Regulation (EC) 854/2004. This study suggests the need for a viral indicator to insure the safety for consumption of shellfish.
本研究的目的是在“实地”评估贝类中病毒的积累情况，并将其与细菌指标的浓度进行比较。将加利福尼亚贻贝个体放置在受污染的沿海海湾的两个采样站以及离岸一公里处的一个对照站。每周对海水和贝类样本中的轮状病毒和大肠杆菌的存在情况进行评估。通过切向流超滤法初步浓缩后，在水中检测到轮状病毒基因组，并通过实时聚合酶链反应在贻贝的肝胰腺中检测到。通过过滤法对水基质中的大肠杆菌进行计数，通过最大可能数法对贻贝中的大肠杆菌进行计数。海水中未记录到轮状病毒，而在贻贝中，自放置后第三周起检测到轮状病毒。贻贝中的大肠杆菌含量始终低于欧盟法规（EC）854/2004规定的限值。本研究表明需要一种病毒指标来确保贝类消费的安全性。
