Divizia M, Santi A L, Panà A
Department of Public Health, Second University of Rome, Italy.
J Virol Methods. 1989 Jan;23(1):55-62. doi: 10.1016/0166-0934(89)90089-x.
The efficiency of seeded hepatitis A virus and poliovirus recovery from 1 l of dechlorinated tap water or different buffer was evaluated using a molecular filtration system. All the experiments were performed using a polysulfonate membrane of 10,000 molecular weight limit. Under standard conditions hepatitis A virus recovery was 100% of the input, but the percentage was reduced dramatically when the inflow pressure was increased. In contrast, poliovirus recovery was low under standard conditions, but it improved when the membranes were pretreated with different buffers. The best recovery was obtained using beef extract at neutral pH.
使用分子过滤系统评估了从1升脱氯自来水或不同缓冲液中回收甲型肝炎病毒和脊髓灰质炎病毒的效率。所有实验均使用分子量截留值为10,000的聚磺酸盐膜进行。在标准条件下，甲型肝炎病毒的回收率为输入量的100%，但当进水压力增加时，该百分比会急剧下降。相比之下，脊髓灰质炎病毒在标准条件下的回收率较低，但当用不同缓冲液对膜进行预处理时，回收率会提高。在中性pH值下使用牛肉浸出液可获得最佳回收率。
