Yegorova Alla, Karasyov Alexander, Duerkop Axel, Ukrainets Igor, Antonovich Valery
AV Bogatsky Physico-Chemical Institute of the National Academy of Sciences of Ukraine, 86, Lustdorfskaya doroga, Odessa 65080, Ukraine.
Spectrochim Acta A Mol Biomol Spectrosc. 2005 Jan 1;61(1-2):109-16. doi: 10.1016/j.saa.2004.03.019.
New terbium complexes of derivatives of 2-oxo-4-hydroxy-quinoline-3-carboxylic acid are reported, which are highly luminescent, water soluble and do not require luminescence enhancers. The triplet-state energy levels of the ligands, the relative quantum yields (QYs) and the excitation maxima of the respective terbium chelates were determined. The large luminescence enhancement of one of these complexes by nucleic acids was investigated and a mechanism of its interaction with DNA is proposed. The optimal conditions for determination of DNA are equal concentrations of Tb(3+) and ligand R(1) (C = 1 x 10(-6) M), pH 9.0. Under optimal conditions the luminescence intensity (RI) is proportional to the concentration of fish sperm DNA (fsDNA) or calf thymus DNA (ctDNA), respectively, within the range of 0.05-1.5 microg ml(-1). The detection limits were 10 ng ml(-1) for fsDNA and 12 ng ml(-1) for ctDNA.
报道了2-氧代-4-羟基喹啉-3-羧酸衍生物的新型铽配合物，它们具有高发光性、水溶性且不需要发光增强剂。测定了配体的三重态能级、相应铽螯合物的相对量子产率（QYs）和激发最大值。研究了其中一种配合物被核酸大幅增强发光的情况，并提出了其与DNA相互作用的机制。测定DNA的最佳条件是铽（3+）和配体R（1）浓度相等（C = 1×10⁻⁶ M），pH值为9.0。在最佳条件下，发光强度（RI）分别与鱼精DNA（fsDNA）或小牛胸腺DNA（ctDNA）的浓度在0.05 - 1.5微克/毫升范围内成正比。fsDNA的检测限为10纳克/毫升，ctDNA的检测限为12纳克/毫升。
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