使用铽（III）-二氟沙星配合物对DNA进行灵敏的发光测定。

Sensitive luminescent determination of DNA using the terbium(III)-difloxacin complex.

作者信息

Yegorova Alla V, Scripinets Yulia V, Duerkop Axel, Karasyov Alexander A, Antonovich Valery P, Wolfbeis Otto S

机构信息

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, Germany.

出版信息

Anal Chim Acta. 2007 Feb 19;584(2):260-7. doi: 10.1016/j.aca.2006.11.065. Epub 2006 Dec 3.

DOI:10.1016/j.aca.2006.11.065

PMID:17386613

Abstract

The interaction of the terbium-difloxacin complex (Tb-DFX) with DNA has been examined by using UV-vis absorption and luminescence spectroscopy. The Tb-DFX complex shows an up to 85-fold enhancement of luminescence intensity upon titration with DNA. The long decay times allow additional detection schemes like time-resolved measurements in microplate readers to enhance sensitivity by off-gating short-lived background luminescence. Optimal conditions are found at equimolar concentrations of Tb(3+) and DFX (0.1 or 1 microM) at pH 7.4. Under these conditions, the luminescence intensity is linearly dependent on the concentration of ds-DNAs and ss-DNA between 1-1500 ng mL(-1) and 4.5-270 ng mL(-1), respectively. The detection limit is 0.5 ng mL(-1) for ds-DNAs and 2 ng mL(-1) for ss-DNA. The mechanism for the luminescence enhancement was also studied.

摘要

通过紫外可见吸收光谱和发光光谱研究了铽-二氟沙星络合物（Tb-DFX）与DNA的相互作用。用DNA滴定后，Tb-DFX络合物的发光强度增强了85倍。较长的衰减时间允许采用额外的检测方案，如在微孔板读数器中进行时间分辨测量，通过排除短寿命背景发光来提高灵敏度。在pH 7.4时，Tb(3+)和DFX等摩尔浓度（0.1或1 microM）下发现了最佳条件。在这些条件下，发光强度分别与ds-DNAs和ss-DNA的浓度在1-1500 ng mL(-1)和4.5-270 ng mL(-1)之间呈线性关系。ds-DNAs的检测限为0.5 ng mL(-1)，ss-DNA的检测限为2 ng mL(-1)。还研究了发光增强的机制。