β-防御素mRNA（舌抗菌肽）在牛乳腺上皮组织中的表达受乳腺炎诱导。
Expression of a beta-defensin mRNA, lingual antimicrobial peptide, in bovine mammary epithelial tissue is induced by mastitis.
作者信息
Swanson Kara, Gorodetsky Stas, Good Laura, Davis Stephen, Musgrave David, Stelwagen Kerst, Farr Vicki, Molenaar Adrian
机构信息
Dairy Biotechnology, AgResearch, Hamilton, New Zealand.
出版信息
Infect Immun. 2004 Dec;72(12):7311-4. doi: 10.1128/IAI.72.12.7311-7314.2004.
Abstract
The expression of a beta-defensin, the lingual antimicrobial peptide (LAP), in response to mastitis was investigated by real-time PCR of RNA from mastitic and control udder quarters. There was a positive relationship between somatic cell count in milk and LAP expression. In situ hybridization showed that LAP mRNA was expressed in epithelial cells of mastitic tissue. These results suggest that LAP plays a role in the innate immune response to mastitis.
摘要
通过对患乳腺炎和对照乳腺区的RNA进行实时PCR，研究了一种β-防御素——舌抗菌肽（LAP）在乳腺炎反应中的表达。牛奶中的体细胞计数与LAP表达之间存在正相关关系。原位杂交显示，LAP mRNA在乳腺炎组织的上皮细胞中表达。这些结果表明，LAP在乳腺炎的天然免疫反应中发挥作用。
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本文引用的文献
1
Mastitis increases mammary mRNA abundance of beta-defensin 5, toll-like-receptor 2 (TLR2), and TLR4 but not TLR9 in cattle.
Clin Diagn Lab Immunol. 2004 Jan;11(1):174-85. doi: 10.1128/cdli.11.1.174-185.2004.
2
Abundant human beta-defensin-1 expression in milk and mammary gland epithelium.
J Pediatr. 2001 Jan;138(1):109-12. doi: 10.1067/mpd.2001.109375.
3
Epithelial antibiotic induced in states of disease.
Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8686-90. doi: 10.1073/pnas.94.16.8686.
4
A peptide antibiotic from human skin.
Nature. 1997 Jun 26;387(6636):861. doi: 10.1038/43088.
5
Elevation of lactoferrin gene expression in developing, ductal, resting, and regressing parenchymal epithelium of the ruminant mammary gland.
J Dairy Sci. 1996 Jul;79(7):1198-208. doi: 10.3168/jds.S0022-0302(96)76473-1.
6
Effect of milking frequency on milk somatic cell count characteristics and mammary secretory cell damage in cows.
Am J Vet Res. 1996 Jun;57(6):902-5.
7
Coordinate induction of two antibiotic genes in tracheal epithelial cells exposed to the inflammatory mediators lipopolysaccharide and tumor necrosis factor alpha.
Infect Immun. 1996 May;64(5):1565-8. doi: 10.1128/iai.64.5.1565-1568.1996.
8
Epithelial antibiotics induced at sites of inflammation.
Science. 1995 Mar 17;267(5204):1645-8. doi: 10.1126/science.7886453.
9
Defensins: a family of antimicrobial and cytotoxic peptides.
Toxicology. 1994 Feb 28;87(1-3):131-49. doi: 10.1016/0300-483x(94)90158-9.
10
Bactericidal potency and mechanistic specificity of neutrophil defensins against bovine mastitis pathogens.
Vet Microbiol. 1991 Sep;29(1):49-58. doi: 10.1016/0378-1135(91)90109-s.
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