Gan Miao, Qi Yipeng, Wan Qingwen, Kuang Ersheng, Liu Qingzhen, Liu Xin
Division of Molecular Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Mol Biol Rep. 2004 Sep;31(3):159-64. doi: 10.1023/b:mole.0000043551.84883.c8.
In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis-inducing protein, as a GST-fusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death. The HAP protein inhibited the bacterial growth within 30 min after inducting HAP expression. The transmission electron microscopic examination revealed that the morphology of the bacterial cells expressing hap was changed dramatically: unusually elongated phenotype compared with those of controls and finally leading to cell death. The lethality of HAP was relieved by the addition of vitamin E as a reducing agent and under anaerobic growth conditions. These results suggest that a trace amount of HAP induces bacterial cell death and the death is related with reactive oxygen species (ROS).
在试图产生内质网（ER）靶向凋亡诱导蛋白HAP时，作为一种GST融合蛋白，我们发现HAP的表达而非单独的GST诱导细菌细胞死亡。HAP蛋白在诱导其表达后30分钟内抑制细菌生长。透射电子显微镜检查显示，表达hap的细菌细胞形态发生了显著变化：与对照相比，出现异常伸长的表型，最终导致细胞死亡。作为还原剂的维生素E的添加以及在厌氧生长条件下，HAP的致死性得到缓解。这些结果表明，微量的HAP诱导细菌细胞死亡，且这种死亡与活性氧（ROS）有关。
