Brewer Molly A, Utzinger Urs, Barton Jennifer K, Hoying James B, Kirkpatrick Nathaniel D, Brands William R, Davis John R, Hunt Katherine, Stevens Sally J, Gmitro Arthur F
Biomedical Engineering Program, University of Arizona, 1515 N. Campbell Ave., Room 1968, Tucson, AZ 85724-5024, USA.
Technol Cancer Res Treat. 2004 Dec;3(6):617-27. doi: 10.1177/153303460400300612.
Epithelial ovarian cancer has the highest mortality rate among the gynecologic cancers and spreads beyond the ovary in 90% of the women diagnosed with ovarian cancer. Detection before the disease has spread beyond the ovary would significantly improve the survival from ovarian cancer, which is currently only 30% over 5 years, despite extensive efforts to improve the survival. This study describes initial investigation of the use of optical technologies to improve the outcome for this disease by detecting cancers at an earlier and more treatable stage. Women undergoing oophorectomy were recruited for this study. Ovaries were harvested for fluorescence spectroscopy, confocal microscopy, and optical coherence tomography. Fluorescence spectroscopy showed large diagnostic differences between normal and abnormal tissue at 270 and 340 nm excitation. Optical coherence tomography was able to image up to 2mm deep into the ovary with particular patterns of backscattered intensity observed in normal versus abnormal tissue. Fluorescence confocal microscopy was able to visualize sub-cellular structures of the surface epithelium and underlying cell layers. Optical imaging and/or spectroscopy has the potential to improve the diagnostic capability in the ovary, but extended systematic investigations are needed to identify the unique signatures of disease. The combination of optical technologies supported by modern molecular biology may lead to an instrument that can accurately detect early carcinogenesis.
上皮性卵巢癌在妇科癌症中死亡率最高，90%被诊断为卵巢癌的女性其癌症会扩散至卵巢以外。在疾病扩散至卵巢以外之前进行检测将显著提高卵巢癌患者的生存率，尽管为提高生存率付出了巨大努力，但目前卵巢癌患者的5年生存率仅为30%。本研究描述了对使用光学技术的初步调查，旨在通过在更早且更易治疗的阶段检测癌症来改善这种疾病的治疗效果。本研究招募了接受卵巢切除术的女性。采集卵巢用于荧光光谱分析、共聚焦显微镜检查和光学相干断层扫描。荧光光谱分析显示，在270和340纳米激发波长下，正常组织与异常组织之间存在巨大的诊断差异。光学相干断层扫描能够对卵巢进行高达2毫米深度的成像，在正常组织与异常组织中观察到了特定的背向散射强度模式。荧光共聚焦显微镜能够观察到表面上皮和下层细胞层的亚细胞结构。光学成像和/或光谱分析有潜力提高卵巢疾病的诊断能力，但需要进行更广泛的系统研究来确定疾病的独特特征。由现代分子生物学支持的光学技术组合可能会催生一种能够准确检测早期癌变的仪器。
