Watson Jennifer M, Marion Samuel L, Rice Photini F, Bentley David L, Besselsen David G, Utzinger Urs, Hoyer Patricia B, Barton Jennifer K
Biomedical Engineering; University of Arizona; Tucson, AZ USA.
Physiology Department; University of Arizona; Tucson, AZ USA.
Cancer Biol Ther. 2014 Jan;15(1):42-60. doi: 10.4161/cbt.26605. Epub 2013 Oct 3.
Identification of the early microscopic changes associated with ovarian cancer may lead to development of a diagnostic test for high-risk women. In this study we use optical coherence tomography (OCT) and multiphoton microscopy (MPM) (collecting both two photon excited fluorescence [TPEF] and second harmonic generation [SHG]) to image mouse ovaries in vivo at multiple time points. We demonstrate the feasibility of imaging mouse ovaries in vivo during a long-term survival study and identify microscopic changes associated with early tumor development. These changes include alterations in tissue microstructure, as seen by OCT, alterations in cellular fluorescence and morphology, as seen by TPEF, and remodeling of collagen structure, as seen by SHG. These results suggest that a combined OCT-MPM system may be useful for early detection of ovarian cancer.
识别与卵巢癌相关的早期微观变化可能会促成针对高危女性的诊断测试的开发。在本研究中，我们使用光学相干断层扫描（OCT）和多光子显微镜（MPM）（同时收集双光子激发荧光 [TPEF] 和二次谐波产生 [SHG]）在多个时间点对小鼠卵巢进行体内成像。我们证明了在长期生存研究期间对小鼠卵巢进行体内成像的可行性，并识别出与早期肿瘤发展相关的微观变化。这些变化包括 OCT 所见的组织微观结构改变、TPEF 所见的细胞荧光和形态改变，以及 SHG 所见的胶原结构重塑。这些结果表明，OCT-MPM 联合系统可能有助于卵巢癌的早期检测。
