Kanter Elizabeth M, Walker Ross M, Marion Samuel L, Brewer Molly, Hoyer Patricia B, Barton Jennifer K
The University of Arizona, Division of Biomedical Engineering, Tucson, Arizona 85721, USA.
J Biomed Opt. 2006 Jul-Aug;11(4):041123. doi: 10.1117/1.2236298.
Ovarian cancer is the fifth leading cause of cancer death in women, in part because of the limited knowledge about early stage disease. We develop a novel rat model of ovarian cancer and perform a pilot study to examine the harvested ovaries with complementary optical imaging modalities. Rats are exposed to repeated daily dosing (20 days) with 4-vinylcyclohexene diepoxide (VCD) to cause early ovarian failure (model for postmenopause), and ovaries are directly exposed to 7,12-dimethylbenz(a)anthracene (DMBA) to cause abnormal ovarian proliferation and neoplasia. Harvested ovaries are examined with optical coherence tomography (OCT) and light-induced fluorescence (LIF) at one, three, and five months post-DMBA treatment. VCD causes complete ovarian follicle depletion within 8 months after onset of dosing. DMBA induces abnormal size, cysts, and neoplastic changes. OCT successfully visualizes normal and abnormal structures (e.g., cysts, bursa, follicular remnant degeneration) and the LIF spectra show statistically significant changes in the ratio of average emission intensity at 390:450 nm between VCD-treated ovaries and both normal cycling and neoplastic DMBA-treated ovaries. Overall, this pilot study demonstrates the feasibility of both the novel animal model for ovarian cancer and the ability of optical imaging techniques to visualize ovarian function and health.
卵巢癌是女性癌症死亡的第五大主要原因，部分原因是对早期疾病的了解有限。我们开发了一种新型卵巢癌大鼠模型，并进行了一项初步研究，以使用互补光学成像模式检查收获的卵巢。大鼠每天重复给药（20天）4-乙烯基环己烯二环氧化物（VCD）以导致早期卵巢功能衰竭（绝经后模型），卵巢直接暴露于7,12-二甲基苯并(a)蒽（DMBA）以导致卵巢异常增殖和肿瘤形成。在DMBA治疗后的1、3和5个月，使用光学相干断层扫描（OCT）和光诱导荧光（LIF）检查收获的卵巢。VCD在给药开始后8个月内导致完全的卵巢卵泡耗竭。DMBA诱导大小异常、囊肿和肿瘤变化。OCT成功地可视化了正常和异常结构（例如囊肿、囊、卵泡残余变性），并且LIF光谱显示，在VCD处理的卵巢与正常周期和肿瘤性DMBA处理的卵巢之间，390:450nm处平均发射强度的比率存在统计学上的显著变化。总体而言，这项初步研究证明了新型卵巢癌动物模型以及光学成像技术可视化卵巢功能和健康状况的可行性。
