Jilek Samantha, Merkle Hans P, Walter Elke
Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology Zurich (ETH), Winterthurerstrerstrasse 190, CH-8057 Zurich, Switzerland.
Adv Drug Deliv Rev. 2005 Jan 10;57(3):377-90. doi: 10.1016/j.addr.2004.09.010.
This paper provides a review of the role of dendritic cells (DC) in microparticle-mediated immune response and the advantages of associating DNA to microparticles in order to increase the potency of DNA vaccination in vivo. To begin with, different methods for the preparation of DNA-loaded microparticle with poly(lactide) (PLA)/poly(lactide-co-glycolide) (PLGA) polymers are presented. Further, the effects of DNA-loaded microparticles on DC in vitro are extensively examined including transfection and stimulation of DC, a key feature of the immune response. Finally, in vivo tracking of DNA-loaded microparticles and induction of immune responses upon DNA-loaded microparticle administration in different animal models and with various routes of administration are reviewed.
本文综述了树突状细胞（DC）在微粒介导的免疫反应中的作用，以及将DNA与微粒结合以提高体内DNA疫苗效力的优势。首先，介绍了用聚乳酸（PLA）/聚乳酸-乙醇酸共聚物（PLGA）聚合物制备负载DNA微粒的不同方法。此外，还广泛研究了负载DNA微粒对体外DC的影响，包括DC的转染和刺激，这是免疫反应的一个关键特征。最后，综述了在不同动物模型中通过不同给药途径对负载DNA微粒的体内追踪以及给药后免疫反应的诱导情况。
