Blixt Ola, Head Steve, Mondala Tony, Scanlan Christopher, Huflejt Margaret E, Alvarez Richard, Bryan Marian C, Fazio Fabio, Calarese Daniel, Stevens James, Razi Nahid, Stevens David J, Skehel John J, van Die Irma, Burton Dennis R, Wilson Ian A, Cummings Richard, Bovin Nicolai, Wong Chi-Huey, Paulson James C
Glycan Synthesis and Protein Expression Core-D, Consortium for Functional Glycomics, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17033-8. doi: 10.1073/pnas.0407902101. Epub 2004 Nov 24.
Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.
在此,我们描述了一种聚糖微阵列,它是利用标准的机器人微阵列打印技术构建的,用于将胺功能化聚糖偶联到氨基反应性载玻片上。该阵列包含200个合成和天然聚糖序列,代表糖蛋白和糖脂的主要聚糖结构。该阵列在分析多种聚糖结合蛋白的特异性方面具有显著效用,这些蛋白包括C型凝集素、唾液酸结合免疫球蛋白样凝集素、半乳糖凝集素、抗碳水化合物抗体、来自植物和微生物的凝集素以及完整病毒。