Stenfors L E, Räisänen S
Department of Otolaryngology, Central Hospital of Keski-Pohjanmaa, Kokkola, Finland.
J Laryngol Otol. 1992 Feb;106(2):111-5. doi: 10.1017/s0022215100118833.
Epithelial cells were obtained by swabbing the posterior wall of the nasopharynx (NPH) of 15 patients (age one to 6 8/12 years; eight males, seven females) undergoing ENT-surgery under general anaesthesia for otitis media with effusion (OME). Individually matched, ear healthy children served as controls. Bacteria attached to the non-ciliated cells were calculated according to a method described. Furthermore, quantitative and qualitative bacteriological analyses were performed on samples obtained from mucoid middle ear effusion material as well as from the NPH. All patients and controls harboured middle ear pathogens (S. pneumoniae, H. influenzae, B. catarrhalis, or S. aureus) in the NPH. Only 33 per cent of the patients harboured middle ear pathogens in the middle ear effusion and the same pathogen was invariably found in the corresponding NPH. Attachment of bacteria to the non-ciliated cells of the NPH diminished significantly with growing age in the ear healthy control group but not in the OME group. OME is closely correlated to the presence of middle ear pathogens in the NPH and to attachment of bacteria to the epithelial cells in the NPH.
通过擦拭15名在全身麻醉下接受中耳积液（OME）耳科手术的患者（年龄1至6岁8个月；8名男性，7名女性）的鼻咽后壁（NPH）获取上皮细胞。个体匹配的耳部健康儿童作为对照。根据所述方法计算附着在非纤毛细胞上的细菌数量。此外，对从黏液性中耳积液材料以及NPH获取的样本进行了定量和定性细菌学分析。所有患者和对照在NPH中均携带中耳病原体（肺炎链球菌、流感嗜血杆菌、卡他莫拉菌或金黄色葡萄球菌）。只有33%的患者在中耳积液中携带中耳病原体，且在相应的NPH中总是发现相同的病原体。在耳部健康对照组中，随着年龄增长，细菌附着在NPH的非纤毛细胞上的情况显著减少，但在OME组中并非如此。OME与NPH中中耳病原体的存在以及细菌附着在NPH上皮细胞上密切相关。
