Rezer E, Moulins M
Laboratoire de Neurobiologie et Physiologie Comparées, Université de Bordeaux I, CNRS, Arcachon, France.
J Exp Biol. 1992 Feb;163:209-30. doi: 10.1242/jeb.163.1.209.
In the lobster Jasus lalandii, 14 neurones of the stomatogastric ganglion (STG) are organized in a network that produces rhythmic pyloric outputs. In vitro experiments have shown that the STG neurones receive, via the stomatogastric nerve (stn), neuromodulatory inputs that influence the expression of the bursting properties of the neurones and the ability of the network to produce its rhythmic output. In contrast to these in vitro observations, in vivo transection of the stn does not abolish the pyloric rhythm. Rhythmic output can be recorded by electromyography immediately after stn transection and for up to 2 years afterwards. We have shown that, under these experimental conditions, the STG appears to be isolated from any neuronal input that might account for the maintenance of the rhythmic output. Experiments carried out in the 2 days after stn transection showed that an in vitro preparation of the isolated STG was unable to produce any rhythmic output, but blood serum added to the system could restore the pyloric output. These results suggest strongly that the pyloric network receives neural and humoral modulatory influences in parallel and that each type of influence alone is able to maintain the bursting capability of the pyloric neurones.
在龙虾拉氏岩龙虾（Jasus lalandii）中，口胃神经节（STG）的14个神经元组成一个网络，产生有节律的幽门输出。体外实验表明，STG神经元通过口胃神经（stn）接收神经调节输入，这些输入会影响神经元爆发特性的表达以及网络产生节律性输出的能力。与这些体外观察结果相反，在体内切断stn并不会消除幽门节律。在切断stn后立即通过肌电图可以记录到节律性输出，并且在之后长达2年的时间里都能记录到。我们已经表明，在这些实验条件下，STG似乎与任何可能维持节律性输出的神经元输入隔离。在切断stn后的2天内进行的实验表明，分离的STG体外制备物无法产生任何节律性输出，但添加到系统中的血清可以恢复幽门输出。这些结果强烈表明，幽门网络同时接受神经和体液调节影响，并且每种影响单独都能够维持幽门神经元的爆发能力。
