Lacher N A, Garrison K E, Martin R S, Lunte S M
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66047, USA.
Electrophoresis. 2001 Aug;22(12):2526-36. doi: 10.1002/1522-2683(200107)22:12<2526::AID-ELPS2526>3.0.CO;2-K.
Microfabricated fluidic devices have generated considerable interest over the past ten years due to the fact that sample preparation, injection, separation, derivatization, and detection can be integrated into one miniaturized device. This review reports progress in the development of microfabricated analytical systems based on microchip capillary electrophoresis (CE) with electrochemical (EC) detection. Electrochemical detection has several advantages for use with microchip electrophoresis systems, for example, ease of miniaturization, sensitivity, and selectivity. In this review, the basic components necessary for microchip CEEC are described, including several examples of different detector configurations. Lastly, details of the application of this technique to the determination of catechols and phenols, amino acids, peptides, carbohydrates, nitroaromatics, polymerase chain reaction (PCR) products, organophosphates, and hydrazines are described.
在过去十年中，微纳加工流体装置引起了广泛关注，因为样品制备、进样、分离、衍生化和检测可以集成到一个小型化装置中。本综述报道了基于带有电化学（EC）检测的微芯片毛细管电泳（CE）的微纳加工分析系统的发展进展。电化学检测在微芯片电泳系统中具有几个优点，例如易于小型化、灵敏度高和选择性好。在本综述中，描述了微芯片CE-EC所需的基本组件，包括不同检测器配置的几个示例。最后，描述了该技术在儿茶酚和酚类、氨基酸、肽、碳水化合物、硝基芳烃、聚合酶链反应（PCR）产物、有机磷酸盐和肼的测定中的应用细节。
