Buck C, Paulino G P, Medina D J, Hsiung G D, Campbell T W, Walsh M T
Virology Department, American Type Culture Collection, Rockville, MD 20852, USA.
Clin Diagn Virol. 1993 Jul;1(2):109-12. doi: 10.1016/0928-0197(93)90018-z.
We report the isolation of St. Louis Encephalitis (SLE) virus from a mature male killer whale (Orcinus orca). This represents the first isolation of SLE virus from a marine mammal. The animal presented with reduced appetite, rapidly became lethargic and subsequently died. Virus-induced CPE was observed in a dolphin cell line, SP-1K (ATCC CCL 78), inoculated with brain, kidney, and lung tissues obtained at necropsy. Electron microscopy of infected SP-1K cells revealed the presence of virions having morphology and size resembling members of the Flaviviridae. Final identification as SLE virus was made by neutralization and immunofluorescence staining tests.
