Flø R W, Samdal H H, Kalland K H, Nilsen A, Haukenes G
Medical Department B, The Gade Institute, University of Bergen, Bergen, Norway.
Clin Diagn Virol. 1993 Aug;1(3):143-52. doi: 10.1016/0928-0197(93)90009-t.
Sera from 298 HIV-infected individuals from Southern Norway were examined for antibodies against HTLV. 30 sera (10.1%) were HTLV-II positive and 1(0.3%) HTLV-I positive. 25 of the HTLV-II infected subjects were intravenous drug abusers (IVDAs), giving a prevalence of HTLV-II infection of 24.5% in this group. Examination of blood samples by polymerase chain reaction followed by restriction enzyme analysis or sequencing confirmed the serological diagnosis. To evaluate current screening and verification HTLV tests, 44 sera were examined using a gelatin particle agglutination test, 5 different enzyme-linked immunoassays (ELISA) and 4 Western blots (WB). While earlier ELISAs and WBs were inadequate, a recent ELISA and WB including recombinant envelope glycoproteins from both viruses permitted serological diagnosis and distinction between HTLV-I and HTLV-II. Thus, HTLV-II now spreads among IVDAs in a North-European country. Health authorities in other countries should estimate the magnitude of the problem to decide upon measures to avoid transmission through blood transfusion.
对挪威南部298名HIV感染者的血清进行了抗HTLV抗体检测。30份血清（10.1%）HTLV-II呈阳性，1份血清（0.3%）HTLV-I呈阳性。25名HTLV-II感染受试者为静脉吸毒者，该组HTLV-II感染率为24.5%。通过聚合酶链反应随后进行限制性酶切分析或测序对血样进行检测，证实了血清学诊断。为评估当前的HTLV筛查和验证检测，使用明胶颗粒凝集试验、5种不同的酶联免疫吸附测定（ELISA）和4种免疫印迹法（WB）对44份血清进行了检测。虽然早期的ELISA和WB检测不够充分，但最近一种包括两种病毒重组包膜糖蛋白的ELISA和WB检测能够进行血清学诊断并区分HTLV-I和HTLV-II。因此，HTLV-II目前在一个北欧国家的静脉吸毒者中传播。其他国家的卫生当局应评估该问题的严重程度，以决定采取措施避免通过输血传播。
