Chong C F, Ngoh B L, Tan H C, Yap E H, Singh M, Chan L, Chan Y C
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore City, Singapore 0511.
Clin Diagn Virol. 1994 Jan;1(5-6):335-41. doi: 10.1016/0928-0197(94)90063-9.
A shortened IgM capture ELISA for the detection of dengue IgM antibodies using simultaneous incubation of antigen and peroxidase-labeled monoclonal antibody was described. The shortened two-step assay was compared with the four-step IgM capture ELISA on sera from dengue patients confirmed by the hemagglutination inhibition (HI) test. When paired acute and convalescent sera were tested, the shortened ELISA showed 100% agreement with HI results. It detected dengue IgM antibodies in the acute sera of 66% of patients with a primary dengue infection, 60% of patients with a secondary infection, and 98% of patients with a presumptive secondary infection. When the results of 151 dengue patients were combined, 75% of the acute sera were positive by the shortened IgM capture ELISA.
