Sang C T, Cuzzubbo A J, Devine P L
Department of Pathology, Singapore General Hospital, Singapore.
Clin Diagn Lab Immunol. 1998 Jan;5(1):7-10. doi: 10.1128/CDLI.5.1.7-10.1998.
A commercially available capture enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG antibodies produced during dengue infection (PanBio Dengue Duo) was evaluated with paired serum specimens from 176 patients. Diagnosis was based on a hemagglutination inhibition (HAI) assay, with patients having either primary dengue (n = 90), secondary dengue (n = 58), or no dengue (n = 28) infection. The combined use of IgM and IgG (sensitivity, 99%; specificity, 96%) was superior to the use of IgM alone (sensitivity, 88%; specificity, 96%) or IgG alone (sensitivity, 85%; specificity, 96%). Furthermore, with the first serum sample of the pair of serum samples, the ELISA was able to diagnose significantly more cases of dengue than the HAI assay (55% versus 14%). The results of the IgG capture ELISA gave a significant correlation with those of the HAI assay (r = 0.91; P < 0.0001), and the IgG capture ELISA could be used to distinguish between primary and secondary infection. The best distinction was observed when an IgG cutoff ratio of 3.0 was used, with 88% of primary infections and 98% of secondary infections being correctly classified. This ELISA should prove to be useful in the clinical diagnosis of dengue infection.
采用一种市售的捕获酶联免疫吸附测定法（ELISA）检测登革热感染期间产生的特异性免疫球蛋白M（IgM）和IgG抗体（PanBio登革热双联检测法），对176例患者的配对血清标本进行了评估。诊断基于血凝抑制（HAI）测定法，患者分别患有原发性登革热（n = 90）、继发性登革热（n = 58）或未感染登革热（n = 28）。联合使用IgM和IgG（敏感性为99%；特异性为96%）优于单独使用IgM（敏感性为88%；特异性为96%）或单独使用IgG（敏感性为85%；特异性为96%）。此外，对于配对血清样本中的第一份血清样本，ELISA诊断出的登革热病例显著多于HAI测定法（55%对14%）。IgG捕获ELISA的结果与HAI测定法的结果具有显著相关性（r = 0.91；P < 0.0001），并且IgG捕获ELISA可用于区分原发性感染和继发性感染。当IgG截断比值为3.0时，区分效果最佳，88%的原发性感染和98%的继发性感染被正确分类。这种ELISA在登革热感染的临床诊断中应会被证明是有用的。
