Brumback B G, Cunningham D M, Morris M V, Villavicencio J L
American Medical Laboratories, Inc., 14225 Newbrook Drive, P.O. Box 10841, Chantilly, VA 22021-0841, USA.
Clin Diagn Virol. 1995 Oct;4(3):251-6. doi: 10.1016/0928-0197(95)00015-z.
Rapid diagnosis and typing of influenza virus are important for patient treatment and management during seasonal outbreaks. Centrifugation-enhanced rapid culture has been reported to be useful as an adjunct to traditional tube culture for rapid diagnosis of influenza virus.
We compared rapid culture in 96-well plates against standard tube culture for recovery of influenza virus, types A and B. We also tested two different cell types, MDCK and RMK, to determine if the use of multiple cell lines increases the sensitivity of rapid culture.
The rapid method was initially evaluated by retrospective culture of previously positive frozen specimens. It was then compared to standard culture for recovery of influenza virus by parallel testing of fresh respiratory specimens.
Of 32 previously positive frozen specimens, 28 were positive upon repeat culture. Rapid culture recovered 25 (89.3%) and standard culture recovered 23 (82.1%). All positives were type A. Of 722 fresh specimens cultured in parallel, 76 (10.5%) were positive for influenza virus: 43 for type A and 33 for type B. For type A, rapid culture recovered 42 of 43 (97.7%) and tube culture recovered 39 (90.7%). For type B, rapid culture recovered 33 of 33 (100%) and tube culture recovered 24 (72.7%). In the rapid system, the MDCK cell line was positive for 40 of 42 type A positives (95.2%) and the RMK was positive for 41 (97.6%). The MDCK line was positive for 32 of the 33 type B isolates (97.0%) and the RMK cells were positive for all 33 (100%).
Rapid culture substantially reduced total test time and was more sensitive than tube culture. Duplicate cell lines did not significantly increase test sensitivity.
在季节性流感爆发期间，流感病毒的快速诊断和分型对于患者的治疗和管理至关重要。据报道，离心增强快速培养作为传统试管培养的辅助手段，可用于流感病毒的快速诊断。
我们比较了在96孔板中进行的快速培养与用于甲型和乙型流感病毒回收的标准试管培养。我们还测试了两种不同的细胞类型，MDCK和RMK，以确定使用多种细胞系是否会提高快速培养的敏感性。
快速方法最初通过对先前阳性冷冻标本的回顾性培养进行评估。然后通过对新鲜呼吸道标本的平行检测，将其与用于流感病毒回收的标准培养进行比较。
在32份先前阳性的冷冻标本中，28份在重复培养时呈阳性。快速培养回收了25份（89.3%），标准培养回收了23份（82.1%）。所有阳性均为甲型。在722份平行培养的新鲜标本中，76份（10.5%）流感病毒呈阳性：43份为甲型，33份为乙型。对于甲型，快速培养回收了43份中的42份（97.7%），试管培养回收了39份（90.7%）。对于乙型，快速培养回收了33份中的33份（100%），试管培养回收了24份（72.7%）。在快速系统中，MDCK细胞系对42份甲型阳性中的40份呈阳性（95.2%），RMK对41份呈阳性（97.6%）。MDCK细胞系对33份乙型分离株中的32份呈阳性（97.0%），RMK细胞对所有33份均呈阳性（100%）。
快速培养显著缩短了总检测时间，且比试管培养更敏感。重复使用细胞系并未显著提高检测敏感性。
