Brumback B G, Wade C D
Virology Department, American Medical Laboratories, Inc., Chantilly, Virginia 22021, USA.
J Clin Microbiol. 1996 Apr;34(4):798-801. doi: 10.1128/jcm.34.4.798-801.1996.
A simultaneous rapid culture for influenza virus types A and B, parainfluenza virus, and respiratory syncytial virus was developed in a 96-well plate format with a culture-confirmatory stain using multiple fluorescent tags. Performance characteristics were comparable to those of standard and/or single rapid-culture methods as shown by parallel testing of 590 fresh clinical specimens and retrospective testing of 190 previously positive frozen specimens. The quadruple culture required less specimen volume than separate cultures, was significantly quicker than standard tube culture, was less labor intensive than separate cultures, and was less expensive than the other methods.
采用96孔板形式开发了一种同时快速培养甲型和乙型流感病毒、副流感病毒及呼吸道合胞病毒的方法，该方法使用多种荧光标记进行培养确认染色。对590份新鲜临床标本进行平行检测以及对190份先前阳性的冷冻标本进行回顾性检测，结果表明其性能特征与标准和/或单一快速培养方法相当。四重培养所需标本量比单独培养少，比标准试管培养显著更快，比单独培养劳动强度低，且比其他方法成本低。
