Son Ora, Cho Hee-Yeon, Kim Mi-Ran, Lee Hyosoo, Lee Myeong-Sok, Song Eunsook, Park Jong Hoon, Nam Kyung Hee, Chun Jong-Yoon, Kim Ho-Jung, Hong Soon-Kwan, Chung Yong-Yoon, Hur Cheol-Goo, Cho Hyung-Taeg, Cheon Choong-Ill
Department of Biological Science, Sookmyung Women's University, Seoul, Republic of Korea.
Biochem Biophys Res Commun. 2005 Jan 7;326(1):203-9. doi: 10.1016/j.bbrc.2004.11.014.
Homeobox genes are essential regulators of the development of plants as well as other organisms. We chose eight putative Arabidopsis homeobox genes not previously characterized and examined their expression in response to treatment with auxin/cytokinin. One of them, ATHB53, was further studied because it was auxin-inducible and its induction was inhibited by cytokinin. Its full-length cDNA was cloned and found to encode a protein of the HD-Zip superfamily. Whole-mount in situ hybridization and RT-PCR showed that it was expressed in the root meristem, and auxin treatment increased its expression, especially in a region from 0.3 to 0.6mm from the root tip. These results suggest that ATHB53 plays a regulatory role in auxin/cytokinin signaling during root development.
同源异型盒基因是植物以及其他生物体发育的重要调节因子。我们选择了八个先前未被鉴定的拟南芥假定同源异型盒基因，并检测了它们对生长素/细胞分裂素处理的响应表达。其中一个基因ATHB53，因其受生长素诱导且其诱导被细胞分裂素抑制而被进一步研究。克隆了它的全长cDNA，发现其编码一个HD-Zip超家族的蛋白质。整装原位杂交和RT-PCR表明它在根分生组织中表达，生长素处理增加了它的表达，特别是在根尖0.3到0.6毫米的区域。这些结果表明ATHB53在根发育过程中的生长素/细胞分裂素信号传导中起调节作用。
